Anneal Extend PCR Protocol - for linking 2 pieces of DNA: Difference between revisions

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== Anneal Extend PCR ==
== Anneal Extend PCR ==


This is a protocol for linking any 2 pieces of DNA together using homologous primers.
This is a protocol for linking any 2 pieces of DNA together using homologous primers.


The original protocol can be found here[http://openwetware.org/wiki/Image:Anneal_Extend.pdf].
The original protocol can be found here[http://openwetware.org/images/9/95/Anneal_Extend.pdf].


As well, there is a protocol for introducing mutations via the primer.  That protocol can be found here[http://openwetware.org/images/2/26/Megaprimer_PCR_Protocol.pdf].
As well, there is a protocol for introducing mutations via the primer.  That protocol can be found here[http://openwetware.org/images/2/26/Megaprimer_PCR_Protocol.pdf].
[[Category:Protocol]] [[Category:In vitro]] [[Category:DNA]]

Latest revision as of 10:31, 23 February 2009

back to protocols

Anneal Extend PCR

This is a protocol for linking any 2 pieces of DNA together using homologous primers.

The original protocol can be found here[1].

As well, there is a protocol for introducing mutations via the primer. That protocol can be found here[2].