Anneal Extend PCR Protocol - for linking 2 pieces of DNA: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 3: Line 3:
This is a protocol for linking any 2 pieces of DNA together using homologous primers.
This is a protocol for linking any 2 pieces of DNA together using homologous primers.


The original protocol can be found here[http://openwetware.org/wiki/Image:Anneal_Extend.pdf].
The original protocol can be found here[http://openwetware.org/images/9/95/Anneal_Extend.pdf].


As well, there is a protocol for introducing mutations via the primer.  That protocol can be found here[http://openwetware.org/images/2/26/Megaprimer_PCR_Protocol.pdf].
As well, there is a protocol for introducing mutations via the primer.  That protocol can be found here[http://openwetware.org/images/2/26/Megaprimer_PCR_Protocol.pdf].

Revision as of 15:18, 22 February 2007

Anneal Extend PCR

This is a protocol for linking any 2 pieces of DNA together using homologous primers.

The original protocol can be found here[1].

As well, there is a protocol for introducing mutations via the primer. That protocol can be found here[2].