Annealing primers: Difference between revisions
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Annealing primers can be used as a fast and cheap way to synthesize a short piece of DNA for which you do not have template DNA to PCR from. See [[Synthetic Biology:BioBricks/Part fabrication|part fabrication]] for other ways to make a part (contains [[Synthetic Biology:BioBricks|BioBrick]] specific details). | Annealing primers can be used as a fast and cheap way to synthesize a short piece of DNA for which you do not have template DNA to PCR from. See [[Synthetic Biology:BioBricks/Part fabrication|part fabrication]] for other ways to make a part (contains [[Synthetic Biology:BioBricks|BioBrick]] specific details). | ||
#[[Annealing complementary primers]]<--For pieces of DNA shorter than the limit on primer length. | #[[Annealing complementary primers]]<--For pieces of DNA shorter than the limit on primer length. | ||
#[[Annealing and primer extension]]<--For pieces of DNA longer than the limit on primer | #[[Annealing and primer extension]]<--For pieces of DNA longer than the limit on primer length. | ||
[[Category:Protocol]] | |||
[[Category:DNA]] | |||
[[Category:In vitro]] | |||
Latest revision as of 14:30, 9 October 2007
Annealing primers can be used as a fast and cheap way to synthesize a short piece of DNA for which you do not have template DNA to PCR from. See part fabrication for other ways to make a part (contains BioBrick specific details).
- Annealing complementary primers<--For pieces of DNA shorter than the limit on primer length.
- Annealing and primer extension<--For pieces of DNA longer than the limit on primer length.
