Anthony J. Wavrin Week 3: Difference between revisions
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==Physiological parameters== | ==Physiological parameters== | ||
* | *The concentrations of ammonia used were 29, 44, 61, 66, 78, 90, 96, 114, and 118mM. | ||
**It is interesting to note that at the concentration of 61mM of ammonia, glucose becomes the limiting nutrient. | |||
===Figure 1=== | |||
====A===== | |||
*The X-axis represents the increase in concentration of the ammonia. | |||
*The Y-axis on the left represents the residual ammonia concentration. | |||
*The Y-axis on the right represents the biomass (dry weight). | |||
*The Y-axis on the far right represents the ammonia flux, which is calculated using ammonia concentration, residual ammonia concentration, and biomass. | |||
*As ammonia increased to ammonia saturation, there was an increase in biomass, but stayed relatively constant after ammonia excess (>61mM). | |||
*The residual ammonia concentration sky rockets after 61mM which is expected due to nitrogen excess. | |||
====B==== | |||
*The X-axis represents the increase in concentration of the ammonia. | |||
*The Y-axis on the left represents the CO2 production. | |||
*The Y-axis on the far left represents the O2 usage. | |||
*The Y-axis on the right represents the respiratory quotient, which is CO2 production/ O2 usage. | |||
*Concentrations above 44mM of ammonia have a relatively flat respiratory quotient. | |||
====C==== | |||
=====Left Figure===== | |||
*The X-axis represents the increase in concentration of the ammonia. | |||
*The Y-axis on the left represents the concentration of α-ketogluterate present. | |||
*As the ammonia concentration increases, α-ketogluterate concentration decreases until 61mM. | |||
=====Middle Figure===== | |||
*The X-axis represents the increase in concentration of the ammonia. | |||
*The Y-axis on the left represents the concentration of glutamate present. | |||
*As the ammonia concentration increases, glutamate concentration increases until 61mM. | |||
=====Right Figure===== | |||
*The X-axis represents the increase in concentration of the ammonia. | |||
*The Y-axis on the left represents the concentration of glutamine present. | |||
*As the ammonia concentration increases, glutamine concentration increases continually. | |||
==RNA Expression== |
Revision as of 21:05, 30 January 2013
Introduction
- This article is exploring one of the possible explanations of how nitrogen, used in the form of ammonia in this study, can effect Saccharomyces cerevisiae.
- It is well known that nitrogen is an essential nutrient that can increase growth, as utilized in fertilizer.
- It is hypothesized that the actual influx of nitrogen may cause growth instead of the concentration.
- This study tests explores if increasing concentration of ammonia while keeping a constant influx will cause nitrogen related responses.
- The concentrations used resulted in testing from nitrogen limitation to nitrogen excess.
- Overall, they conduct effects of physiological parameters, RNA expression, and enzyme expression.
Physiological parameters
- The concentrations of ammonia used were 29, 44, 61, 66, 78, 90, 96, 114, and 118mM.
- It is interesting to note that at the concentration of 61mM of ammonia, glucose becomes the limiting nutrient.
Figure 1
A=
- The X-axis represents the increase in concentration of the ammonia.
- The Y-axis on the left represents the residual ammonia concentration.
- The Y-axis on the right represents the biomass (dry weight).
- The Y-axis on the far right represents the ammonia flux, which is calculated using ammonia concentration, residual ammonia concentration, and biomass.
- As ammonia increased to ammonia saturation, there was an increase in biomass, but stayed relatively constant after ammonia excess (>61mM).
- The residual ammonia concentration sky rockets after 61mM which is expected due to nitrogen excess.
B
- The X-axis represents the increase in concentration of the ammonia.
- The Y-axis on the left represents the CO2 production.
- The Y-axis on the far left represents the O2 usage.
- The Y-axis on the right represents the respiratory quotient, which is CO2 production/ O2 usage.
- Concentrations above 44mM of ammonia have a relatively flat respiratory quotient.
C
Left Figure
- The X-axis represents the increase in concentration of the ammonia.
- The Y-axis on the left represents the concentration of α-ketogluterate present.
- As the ammonia concentration increases, α-ketogluterate concentration decreases until 61mM.
Middle Figure
- The X-axis represents the increase in concentration of the ammonia.
- The Y-axis on the left represents the concentration of glutamate present.
- As the ammonia concentration increases, glutamate concentration increases until 61mM.
Right Figure
- The X-axis represents the increase in concentration of the ammonia.
- The Y-axis on the left represents the concentration of glutamine present.
- As the ammonia concentration increases, glutamine concentration increases continually.