Antibodies
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[[Image:Antibody schematic.jpg|thumb|right|200px|Drawing of an antibody - heavy chains red, light chains yellow.]] | [[Image:Antibody schematic.jpg|thumb|right|200px|Drawing of an antibody - heavy chains red, light chains yellow.]] | ||
| - | == | + | == General == |
* [[Griffin:Antibody Basics]] - Korey's introduction to antibodies | * [[Griffin:Antibody Basics]] - Korey's introduction to antibodies | ||
| - | * [[User:Korey_Griffin | + | * [http://en.wikipedia.org/wiki/Antibody Antibody entry in the Wikipedia] |
| + | |||
| + | == Antibody protocols == | ||
| + | ===[[User:Korey_Griffin|Korey]]'s treasure trove of antibody-related protocols=== | ||
| + | *[[Griffin:Antibody_Basics|Antibody Basics]] | ||
| + | *[[Griffin:Antibody_Peptide_Neutralization|Antibody Peptide Neutralization]] | ||
| + | *[[Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios|Antibody Protein Coupling (F:P) Ratios]] | ||
| + | *[[Griffin:Antibody_Elution_Buffers|Antibody Elution Buffers]] | ||
| + | *[[Griffin:Antibody_Related_Solutions_%26_Recipes|Antibody Related Solutions & Recipes]] | ||
| + | *[[Griffin:Antigen_Retrieval_Technique|Antigen Retrieval Technique]] | ||
| + | *[[Griffin:Chromatin_Immunoprecipitation|Chromatin Immunoprecipitation]] | ||
| + | *[[Griffin:Dual_Immunofluorescence_Cell_Staining|Dual Immunofluorescence Cell Staining]] | ||
| + | *[[Griffin:Flow_Cytometry_prep_%26_labeling|Flow Cytometry prep & labeling]] | ||
| + | *[[Griffin:_Ultimate_Immunoprecipitation_Guide|Immunoprecipitation Guide]] | ||
| + | *[[Griffin:Immunohistochemistry_Paraffin|Immunohistochemistry Paraffin]] | ||
| + | *[[Griffin:Immunofluorescence_Cell_Staining|Immunofluorescence Cell Staining]] | ||
| + | *[[Griffin:Membrane_Stripping_and_Reprobing|Membrane Stripping and Reprobing]] | ||
| + | *[[Western_%28Immuno%29_Blotting|Western Blotting Guide]] | ||
| + | *[[Griffin:Western_Blot_Optimization|Western Blot Optimization]] | ||
| + | |||
| + | ===General protocols & pages involving antibodies=== | ||
* [[Generating antibodies]] | * [[Generating antibodies]] | ||
| + | * [[Protein blot (Western)]] | ||
| + | * [[Dot Blot]] | ||
| + | * [[Affinity purification of antibodies]] | ||
| + | |||
| + | == Lab-specific antibody protocols == | ||
| + | * [[Hoatlin:Crosslinking antibodies to beads]] | ||
| + | * [[Sauer:Western blot]] | ||
| + | * [[Silver:Immunofluorescence]] | ||
| + | * [[Wittrup: Protein conjugation with fluorophores]] | ||
| + | * [[Silver: FISH/IF]] | ||
| + | * [[Berglund:Antibody Storage]] | ||
| + | |||
| + | == Other related pages == | ||
| + | * [[Antibodypedia]] | ||
| + | * [[Toolbox/Lecture_17#ELISA|Toolbox lecture including ELISA]] | ||
| + | |||
| + | |||
| + | [[Category:Material]] [[Category:Protein]] | ||
| + | [[Category:Protocol]] | ||
Current revision
Contents |
General
- Griffin:Antibody Basics - Korey's introduction to antibodies
- Antibody entry in the Wikipedia
Antibody protocols
Korey's treasure trove of antibody-related protocols
- Antibody Basics
- Antibody Peptide Neutralization
- Antibody Protein Coupling (F:P) Ratios
- Antibody Elution Buffers
- Antibody Related Solutions & Recipes
- Antigen Retrieval Technique
- Chromatin Immunoprecipitation
- Dual Immunofluorescence Cell Staining
- Flow Cytometry prep & labeling
- Immunoprecipitation Guide
- Immunohistochemistry Paraffin
- Immunofluorescence Cell Staining
- Membrane Stripping and Reprobing
- Western Blotting Guide
- Western Blot Optimization
General protocols & pages involving antibodies
Lab-specific antibody protocols
- Hoatlin:Crosslinking antibodies to beads
- Sauer:Western blot
- Silver:Immunofluorescence
- Wittrup: Protein conjugation with fluorophores
- Silver: FISH/IF
- Berglund:Antibody Storage
