Antibody:32-4800 mouse monoclonal PTBP1

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Current revision (13:20, 13 September 2010) (view source)
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== Comparison immunofluorescence of several Ptbp1 antibodies ==
== Comparison immunofluorescence of several Ptbp1 antibodies ==
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[[Image:Comparison IF Ptbp1 antibodies.jpg|thumb|400px|left|Comparison of immunofluorescence on mouse pancreatic islet cryosections. Invitrogen/Zymed Ptbp1 antibody bottom right. Similar IF quality to Lifespan (top left). Much better than other 2 antibodies.]]
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[[Image:Comparison IF Ptbp1 antibodies.jpg|400px]]<br>
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Comparison of immunofluorescence on mouse pancreatic islet cryosections. Invitrogen/Zymed Ptbp1 antibody bottom right. Similar IF quality to Lifespan (top left). Much better than other 2 antibodies.
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== Cell line IF ==
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[[Image:PTBP1 goat antibody Everest vs Zymed INS1 PFA fixed GSDB.jpg|500px]]
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Antibody labelling of the same batch of INS1 cell line with the PTBP1 antibody from Zymed, now sold by Invitrogen, shows the typical nuclear labelling of PTBP1. The [[Goat PTBP1 antibody (EB06088)|Everest goat antibody (middle)]] showed no specific staining above the unspecific fluorescence also observed in the no primary antibody control (right).
== Similar antibodies ==
== Similar antibodies ==
* [[Antibody:LS-C38296 mouse monoclonal PTBP1]]
* [[Antibody:LS-C38296 mouse monoclonal PTBP1]]
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* [[Goat PTBP1 antibody (EB06088)]]

Current revision

                       
List of OpenWetWare Antibodies
anti PTBP1 32-4800 Invitrogen (Zymed)
Antibody Type  mouse monoclonal
Target UniProt ID  PTBP1_HUMAN
Cross-Reactive w  mouse, rat
Not Cross-Reactive w  ?
Raised Against  human, recombinant, full-length PTBP1
Provider  Invitrogen (formerly Zymed)
Suitability for..
Western Blot  worked
Immunoprecipitation  worked
Staining fixed cells   worked
Staining cryo-sections  worked
Staining paraffin sections  untested
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Comparison immunofluorescence of several Ptbp1 antibodies


Comparison of immunofluorescence on mouse pancreatic islet cryosections. Invitrogen/Zymed Ptbp1 antibody bottom right. Similar IF quality to Lifespan (top left). Much better than other 2 antibodies.

Cell line IF

Antibody labelling of the same batch of INS1 cell line with the PTBP1 antibody from Zymed, now sold by Invitrogen, shows the typical nuclear labelling of PTBP1. The Everest goat antibody (middle) showed no specific staining above the unspecific fluorescence also observed in the no primary antibody control (right).

Similar antibodies

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