Antibody:32-4800 mouse monoclonal PTBP1: Difference between revisions
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== Comparison immunofluorescence of several Ptbp1 antibodies == | == Comparison immunofluorescence of several Ptbp1 antibodies == | ||
[[Image:Comparison IF Ptbp1 antibodies.jpg | [[Image:Comparison IF Ptbp1 antibodies.jpg|400px]]<br> | ||
Comparison of immunofluorescence on mouse pancreatic islet cryosections. Invitrogen/Zymed Ptbp1 antibody bottom right. Similar IF quality to Lifespan (top left). Much better than other 2 antibodies. | |||
== Cell line IF == | |||
[[Image:PTBP1 goat antibody Everest vs Zymed INS1 PFA fixed GSDB.jpg|500px]] | |||
Antibody labelling of the same batch of INS1 cell line with the PTBP1 antibody from Zymed, now sold by Invitrogen, shows the typical nuclear labelling of PTBP1. The [[Goat PTBP1 antibody (EB06088)|Everest goat antibody (middle)]] showed no specific staining above the unspecific fluorescence also observed in the no primary antibody control (right). | |||
== Similar antibodies == | == Similar antibodies == | ||
* [[Antibody:LS-C38296 mouse monoclonal PTBP1]] | * [[Antibody:LS-C38296 mouse monoclonal PTBP1]] | ||
* [[Goat PTBP1 antibody (EB06088)]] | * [[Goat PTBP1 antibody (EB06088)]] | ||
Latest revision as of 10:20, 13 September 2010
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| Antibody Type | mouse monoclonal |
| Target UniProt ID | PTBP1_HUMAN |
| Cross-Reactive w | mouse, rat |
| Not Cross-Reactive w | ? |
| Raised Against | human, recombinant, full-length PTBP1 |
| Provider | Invitrogen (formerly Zymed) |
| Suitability for.. | |
| Western Blot | worked |
| Immunoprecipitation | worked |
| Staining fixed cells | worked |
| Staining cryo-sections | worked |
| Staining paraffin sections | untested |
| Help | |
Comparison immunofluorescence of several Ptbp1 antibodies
Comparison of immunofluorescence on mouse pancreatic islet cryosections. Invitrogen/Zymed Ptbp1 antibody bottom right. Similar IF quality to Lifespan (top left). Much better than other 2 antibodies.
Cell line IF
Antibody labelling of the same batch of INS1 cell line with the PTBP1 antibody from Zymed, now sold by Invitrogen, shows the typical nuclear labelling of PTBP1. The Everest goat antibody (middle) showed no specific staining above the unspecific fluorescence also observed in the no primary antibody control (right).
