Arabinose - Revision history

Janet B. Matsen: /* Procurement */

Janet B. Matsen — Tue, 30 Oct 2012 20:24:14 GMT

Procurement

←Older revision		Revision as of 20:24, 30 October 2012
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	==Procurement==		==Procurement==
	*You can buy Arabinose for example at [http://www.sigmaaldrich.com Sigma].		*You can buy Arabinose for example at [http://www.sigmaaldrich.com Sigma].
		+	*The chirality of arabinose used for induction is very important. '''L-arabinose works great, but D-arabinose doesn't induce at all'''. L-arabinose is available from Sigma (catalog# A3256).

	==Use==		==Use==

Janet B. Matsen: /* Purpose */

Janet B. Matsen — Tue, 30 Oct 2012 19:19:59 GMT

Purpose

←Older revision		Revision as of 19:19, 30 October 2012
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	==Purpose==		==Purpose==
-	*In biology, Arabinose is often used to induce the P<sub>BAD</sub> promoter described [http://www.~~ncbi~~.~~nlm.nih.gov~~/~~entrez~~/~~query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12080425&query_hl=3~~ here].	+	*In biology, Arabinose is often used to induce the P<sub>BAD</sub> promoter described [http://www.springerlink.com/content/up628e10913tub7u/ here].
-	*Note that P<sub>BAD</sub> is induced by '''L'''-arabinose	+	*Note that P<sub>BAD</sub> is induced by '''L'''-arabinose

	==Procurement==		==Procurement==

Etienne Robillard: /* Use */

Etienne Robillard — Fri, 14 Sep 2012 00:34:55 GMT

Use

←Older revision		Revision as of 00:34, 14 September 2012
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	*MW = 150.14		*MW = 150.14
	*Concentrations in the range 0.001% - 1% are typically used to produce a range of transcription rates from the P<sub>BAD</sub> promoter.		*Concentrations in the range 0.001% - 1% are typically used to produce a range of transcription rates from the P<sub>BAD</sub> promoter.
-	*Note that Arabinose is toxic to ara mutant strains that have araD only ([http://www.ncbi.nlm.nih.gov/pubmed/13890280?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Englesberg et al., 1962]). This is for ~~examlpe~~ the case in MC4100. See [[E. coli genotypes]] for other strains.	+	*Note that Arabinose is toxic to ara mutant strains that have araD only ([http://www.ncbi.nlm.nih.gov/pubmed/13890280?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Englesberg et al., 1962]). This is for example the case in MC4100. See [[E. coli genotypes]] for other strains.

	'''10% w/w Arabinose Stock Solution:'''		'''10% w/w Arabinose Stock Solution:'''

Scott W. Nelson at 14:17, 20 October 2008

Scott W. Nelson — Mon, 20 Oct 2008 14:17:22 GMT

←Older revision		Revision as of 14:17, 20 October 2008
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	==Safety==		==Safety==
-	*Arabinose is just a ~~suggar~~ and harmless.	+	*Arabinose is just a sugar and harmless.

	==References==		==References==

Torsten Waldminghaus: adapted to template and extended content

Torsten Waldminghaus — Tue, 11 Mar 2008 12:26:33 GMT

adapted to template and extended content

←Older revision		Revision as of 12:26, 11 March 2008
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-	==~~General Information~~==	+	==Purpose==
-	~~A few general details about arabinose can be found on the wikipedia [[Wikipedia:Arabinose\|page]].~~ In biology, ~~arabinose~~ is often used to induce the P<sub>BAD</sub> promoter described [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12080425&query_hl=3 here]. ~~Concentrations in the range 0.001% - 1% are typically used to produce a range of transcription rates from the~~ P<sub>BAD</sub> ~~promoter.~~	+	*In biology, Arabinose is often used to induce the P<sub>BAD</sub> promoter described [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12080425&query_hl=3 here].
		+	*Note that P<sub>BAD</sub> is induced by '''L'''-arabinose

-	== ~~10% w/w Arabinose Stock Solution~~==	+	==Procurement==
-	*Arabinose MW = 150.14	+	*You can buy Arabinose for example at [http://www.sigmaaldrich.com Sigma].
		+
		+	==Use==
		+	*MW = 150.14
		+	*Concentrations in the range 0.001% - 1% are typically used to produce a range of transcription rates from the P<sub>BAD</sub> promoter.
		+	*Note that Arabinose is toxic to ara mutant strains that have araD only ([http://www.ncbi.nlm.nih.gov/pubmed/13890280?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Englesberg et al., 1962]). This is for examlpe the case in MC4100. See [[E. coli genotypes]] for other strains.
		+
		+	'''10% w/w Arabinose Stock Solution:'''
	*Add 1g of Arabinose to 9ml of milliQ water in a small falcon tube.		*Add 1g of Arabinose to 9ml of milliQ water in a small falcon tube.
	*Filter sterilize with a 0.22μm filter.		*Filter sterilize with a 0.22μm filter.
	*Can be stored at room temperature.		*Can be stored at room temperature.
		+
		+	==Safety==
		+	*Arabinose is just a suggar and harmless.
		+
		+	==References==
		+	*A few general details about arabinose can be found on the wikipedia [[Wikipedia:Arabinose\|page]].
		+
		+	*[http://www.ncbi.nlm.nih.gov/pubmed/7608087?ordinalpos=8&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum ] Guzman LM, Belin D, Carson MJ, Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol. 1995 Jul;177(14):4121-30

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Reshma P. Shetty at 17:26, 19 October 2007

Reshma P. Shetty — Fri, 19 Oct 2007 17:26:17 GMT

←Older revision		Revision as of 17:26, 19 October 2007
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	*Filter sterilize with a 0.22μm filter.		*Filter sterilize with a 0.22μm filter.
	*Can be stored at room temperature.		*Can be stored at room temperature.
		+
		+	[[Category:Material]] [[Category:Chemical]]

Barry Canton at 15:03, 21 July 2005

Barry Canton — Thu, 21 Jul 2005 15:03:21 GMT

New page

==General Information==
A few general details about arabinose can be found on the wikipedia [[Wikipedia:Arabinose|page]]. In biology, arabinose is often used to induce the P<sub>BAD</sub> promoter described [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12080425&query_hl=3 here]. Concentrations in the range 0.001% - 1% are typically used to produce a range of transcription rates from the P<sub>BAD</sub> promoter.

== 10% w/w Arabinose Stock Solution==
*Arabinose MW = 150.14
*Add 1g of Arabinose to 9ml of milliQ water in a small falcon tube.
*Filter sterilize with a 0.22μm filter.
*Can be stored at room temperature.