Arking:JCAOligoTutorial3

Basic cloning: How to implement the Construction File

This tutorial describes what we'll be doing in the lab as a demonstration of basic cloning. This procedure describes what you'll be doing and seeing during cloning. There's no quiz at the end, but you'll have plenty of real-life experience with this procedure. First of all, let's examine the construction file:

 Construction of GFP Biobrick 2.0 basic part
 PCR ca1123F/ca1123R on pSB1A2-I13522      (748 bp, BglII/XhoI/DpnI)