Keeping track of construction files
You'll be doing lots of cloning--so much so that you probably won't remember how you made any particular constructs 6 months from now. It is therefore imperative that you keep some sort of log that shows what you've made and how you made it. It doesn't really matter how you do it, but here are some suggestions:
The basic construction file
You've already been introduced to the basic construction file in the tutorial which looked like this:
Construction of KanR Basic Part Bca9128 PCR ca1067F/R on pSB1AK3-b0015 (1055bp, EcoRI/SpeI/DpnI) Sub into pSB1A2-I13521 (EcoRI/SpeI, 2062+946, L) Product is pSB1A2-Bca9128 [KanR] ------------------------------------- ca1067F Forward Biobricking of KanR of pSB1AK3 ccagtGAATTCgtccTCTAGAgagctgatccttcaactc ca1067R Reverse Biobricking of KanR of pSB1AK3 gcagtACTAGTtccgtcaagtcagcgtaatg
Always do something like this if you do anything fancy with your cloning like SOEing, restriction site removal, fill ins, blunting, etc. Basically, if it isn't just a PCR, cut, and paste, do this style.