Arking:JCAOligoTutorialHome

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{| class="wikitable" border="0" cellpadding="5" cellspacing="1" style="text-align:center"
{| class="wikitable" border="0" cellpadding="5" cellspacing="1" style="text-align:center"
!width="25%" align="left" valign="top" style="background:#2171B8; color:white"|
!width="25%" align="left" valign="top" style="background:#2171B8; color:white"|
<span style='font-size:18.0pt'>
<span style='font-size:18.0pt'>
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Basic Cloning and Biobrick Assembly Tutorial<br>
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Synthetic Biology Tutorial<br>
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</span>
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<ul>
<ul>
<li>[[User:JCAnderson| Home]]</li>
<li>[[User:JCAnderson| Home]]</li>
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<li>[[Arking:BerkelyiGEm | iGEM]]</li>
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<li>[[Arking:BerkelyiGEm | Teaching]]</li>
<li >[[Arking:JCAProtocols|Protocols]]</li>
<li >[[Arking:JCAProtocols|Protocols]]</li>
<li id="current">[[Arking:JCAOligoTutorialHome | Tutorials]]</li>
<li id="current">[[Arking:JCAOligoTutorialHome | Tutorials]]</li>
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<br>
<br>
<br>
<br>
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==Lectures==
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If you'd like a more advanced series of topics, you might check out my video lectures:
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http://www.youtube.com/channel/UCSVDJJWZ77tOzwl4L9nyg2w/videos?view=1&sort=da&flow=list
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==The Basics==
==The Basics==
'''Think the "Central Dogma" involves a kennel of chihuahuas?  You need to start here with the basics.'''
'''Think the "Central Dogma" involves a kennel of chihuahuas?  You need to start here with the basics.'''
<span style='font-size:18.0pt'>
<span style='font-size:18.0pt'>
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[http://andersonlab.qb3.berkeley.edu/Tutorials/Bare_Bones_Biochemistry.htm Bare bones biochemistry]
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[[Media:Bare_Bones_Biochemistry.pdf]]
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</span>
<br>
<br>
<br>
<br>
<span style='font-size:18.0pt'>
<span style='font-size:18.0pt'>
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[http://andersonlab.qb3.berkeley.edu/Tutorials/Basic_Molecular_Biology.htm Basic Molecular Biology]
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[http://andersonlab.qb3.berkeley.edu/Tutorials/Basic_Molecular_Biology.pdf Basic Molecular Biology]
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<br>
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==Technical Design==
==Technical Design==
<span style='font-size:18.0pt'>
<span style='font-size:18.0pt'>
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[[Arking:JCAOligoTutorial1 | Basic Oligo Design]]
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[[Arking:JCAOligoTutorial1b | Basic Oligo Design]]
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[[Arking:JCAOligoTutorial1 | (old)]]
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<br>
<br>
<br>
<br>
<span style='font-size:18.0pt'>
<span style='font-size:18.0pt'>
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[[Arking:JCAOligoTutorial2 | Special Case: Single Internal Restriction Sites]]
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[[Arking:JCAOligoTutorial2b | Special Case: Single Internal Restriction Sites]]
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[[Arking:JCAOligoTutorial2 | (old)]]
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</span>
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<br>
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<br>
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[[Arking:JCAOligoTutoria21 | Construction of short parts]]
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<span style='font-size:18.0pt'>
[[Arking:JCAOligoTutorial6 | Sequencing Analysis]]
[[Arking:JCAOligoTutorial6 | Sequencing Analysis]]
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</span>
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<br>
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<br>
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==Advanced Technical Design==
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<span style='font-size:18.0pt'>
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[[Arking:JCAOligoTutoria20 | Working with Translational Fusions]]
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</span>
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<br>
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<br>
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<span style='font-size:18.0pt'>
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[[Arking:JCAOligoTutoria22 | Circularly Permuted Proteins]]
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</span>
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<br>
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<br>
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<span style='font-size:18.0pt'>
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[[Arking:JCAOligoTutoria23 | The "what did they make" game]]
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[[Arking:JCAOligoTutorial3 | Implementing the Basic Construction File]]
[[Arking:JCAOligoTutorial3 | Implementing the Basic Construction File]]
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</span>
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<br>
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<br>
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==Introduction to Structural Biology and Part Engineering==
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<span style='font-size:18.0pt'>
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[[Arking:JCAOligoTutoria24 | DeepView Basics]]
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</span>
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<br>
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<br>
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<span style='font-size:18.0pt'>
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[[Arking:JCAOligoTutoria25 | Tour of Structural Biology]]
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----
[[Retired Sections]]<br>
[[Retired Sections]]<br>
[[Arking:JCAOligoTutorialToBeAdded | To Be Added]]
[[Arking:JCAOligoTutorialToBeAdded | To Be Added]]
{{Template:JCA_Arkin_tutorialfooter}}<br>
{{Template:JCA_Arkin_tutorialfooter}}<br>
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[[Arking:JCAOligoTutoria26 | . ]]
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[[Arking:JCAOligoTutoria27 | . ]]
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[[Arking:JCAOligoTutoria28 | . ]]

Current revision

Synthetic Biology Tutorial



Lectures

If you'd like a more advanced series of topics, you might check out my video lectures: http://www.youtube.com/channel/UCSVDJJWZ77tOzwl4L9nyg2w/videos?view=1&sort=da&flow=list

The Basics

Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics.

Media:Bare_Bones_Biochemistry.pdf

Basic Molecular Biology

Practical DNA

What is PCR?

Introduction to Biobricks and Basic Cloning

This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it.

Installing ApE

Overview of Cloning

Introduction to Biobricks

Introduction to Refactoring

Technical Design

Basic Oligo Design (old)

Special Case: Single Internal Restriction Sites (old)

Construction of short parts

Design of composite parts

Keeping track of construction files

Sequencing Analysis

Advanced Technical Design

Working with Translational Fusions

Circularly Permuted Proteins

The "what did they make" game

Implementation

Implementing the Basic Construction File

Introduction to Structural Biology and Part Engineering

DeepView Basics

Tour of Structural Biology

Things you should know

Common Pitfalls and Tips

Origins of replication

The Critical Path



Retired Sections
To Be Added


If you have any comments or want to report a potential error in the tutorial, please email me (Chris Anderson) at JCAnderson2167-at-gmail.com

. . .

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