Arking:JCAOligoTutorialHome

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Introduction to Biobricks and Basic Cloning

This tutorial is best used in conjunction with the sequence editor ApE (A plasmid Editor). It can be downloaded from http://www.biology.utah.edu/jorgensen/wayned/ape/

Introduction to Biobricks

Experimental Design

Basic Oligo Design

Special Case: Single Internal Restriction Sites

Sequencing Analysis

Design of composite parts

Experimental Implementation

Implementing the Basic Construction File

DBBS Composite Part Assembly

If you have any comments or want to report a potential error in the tutorial, please email me (Chris Anderson) at JCAnderson2167-at-gmail.com