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Basic Cloning and Biobrick Assembly Tutorial

The Basics

Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics.

Bare bones biochemistry

Basic Molecular Biology

Practical DNA

What is PCR?

Introduction to Biobricks and Basic Cloning

This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it.

Installing ApE

Overview of Cloning

Introduction to Biobricks

Introduction to Refactoring

Technical Design

Basic Oligo Design

Special Case: Single Internal Restriction Sites

Design of composite parts

Keeping track of construction files

Sequencing Analysis


Implementing the Basic Construction File

Things you should know

Common Pitfalls and Tips

Origins of replication

The Critical Path
Retired Sections
To Be Added

If you have any comments or want to report a potential error in the tutorial, please email me (Chris Anderson) at

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