Basic Cloning and Biobrick Assembly Tutorial
Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics.
Introduction to Biobricks and Basic Cloning
This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it.
Basic Oligo Design
Special Case: Single Internal Restriction Sites
Construction of short parts
Working with Translational Fusions
Design of composite parts
Keeping track of construction files
Circularly Permuted Proteins
Things you should know
If you have any comments or want to report a potential error in the tutorial, please email me (Chris Anderson) at JCAnderson2167-at-gmail.com