Arking:JCATutorialIntro9

Before we start, you will need ApE (A Plasmid Editor). This is a free piece of software that allows you to view, annotate and edit sequence files.

Go to http://www.biology.utah.edu/jorgensen/wayned/ape/ and download the zip file. Open up the file, and install away. The other thing you should do is replace the feature annotation file within ApE with an updated version. To do this, find the directory on your computer in which you installed ApE and locate the file "Default_Features.txt". On my computer, it's: C:\Program Files\ApE\Accessory Files\Features. Replace this file with This Version (and make the name be "Default_Features.txt" so the program still recognizes it.

The original features file contains a list of common plasmid features like origins of replication and antibiotic resistance genes. The replacement file has a lot more stuff that reflects things that exist in the freezers in the lab and in the Registry of Parts collection.