Arrest in G1 phase using a/alpha Mating Factor: Difference between revisions
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If growing a-type yeast, use alpha mating factor. For alpha-type yeast, use a mating factor. | If growing a-type yeast, use alpha mating factor. For alpha-type yeast, use a mating factor. | ||
3) Incubate at 30 C for 3 hours. The | 3) Incubate at 30 C for 3 hours. The yeast should form "shmoos" and look pear-shaped once arrested. | ||
Revision as of 23:18, 25 February 2006
Protocol
1) Grow yeast cells to desired density.
2) Add yeast mating factor to a final concentration of 2.5 ug/mL.
If growing a-type yeast, use alpha mating factor. For alpha-type yeast, use a mating factor.
3) Incubate at 30 C for 3 hours. The yeast should form "shmoos" and look pear-shaped once arrested.
