Arrest in G1 phase using a/alpha Mating Factor: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 4: | Line 4: | ||
2) Add yeast mating factor to a final concentration of 2.5 ug/mL. | 2) Add yeast mating factor to a final concentration of 2.5 ug/mL. | ||
If growing a-type yeast, use alpha mating factor. For alpha-type yeast, use a mating factor. | |||
3) Incubate at 30 C for 3 hours. The yeast should form "shmoos" and look pear-shaped once arrested. | 3) Incubate at 30 C for 3 hours. The yeast should form "shmoos" and look pear-shaped once arrested. | ||
Latest revision as of 23:20, 25 February 2006
Protocol
1) Grow yeast culture to desired density.
2) Add yeast mating factor to a final concentration of 2.5 ug/mL.
If growing a-type yeast, use alpha mating factor. For alpha-type yeast, use a mating factor.
3) Incubate at 30 C for 3 hours. The yeast should form "shmoos" and look pear-shaped once arrested.
