Article Outline: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 31: | Line 31: | ||
==Results and Discussion== | ==Results and Discussion== | ||
===Renalexin elicits significant changes in transcript and protein levels=== | |||
*Sublethal ranalexin impaired but didn't abolish growth of MRSA-252 at concentration of 20μg/ml. | |||
*Proteome and transcriptome profiling applied between control and renalexin exposed MRSA-252 cultures. Microarrays showed: | |||
**93 upregulated genes. | |||
**105 downregulated genes. | |||
***No inconsistencies shown | |||
Revision as of 15:39, 9 November 2011
Abstract
- Background
- Staphylococcus aureus - human pathogen. Treatments are needed. Antimicrobial peptides potential antibiotic to MRSA.
- Such as Renalexin - fights against gram positive bacteria, S. aureus in particular.
- Study of drug resistance and mode of action is important.
- Staphylococcus aureus - human pathogen. Treatments are needed. Antimicrobial peptides potential antibiotic to MRSA.
- Results
- Bayesian logistic regression - allows identification of renalexin response modules from MRSA-252 preteome and transcriptome profiling.
- Relaxin displayed killing mechanisms & cell wall activity.
- Gene disruption and osmotic fragility experiments supported cell wall effects.
- 22 Virulence factors inferred --> VraRS two-component system & PhoU-mediated persister formation --> MRSA tolerance to cationic antimicrobial peptides.
- Conclusions
- Integrative approach to study drug resistance and their mode of action. Finds lead to development of trategies against Staphylococcus aureus (MRSA in particular)
Background
- MRSA - major cause of mortality and mobility. Resistant strains to treatments continue to emerge. Global problem --> community-associated MRSA.
- Prevention and treatment is needed!
- Antimicrobial peptides (AMPs) - novel antibiotic --> could be developed to combat bacteria that is resistant (such as MRSA).
- Produces by all living creatures for defenses -- 880 have been described.
- Renalexin
- 20 a.a. peptide (cationic)
- Possesses single intramolecular dispulphide bond --> forming heptapeptide ring at carboxyl terminus.
- Displays strong activity against Staphylococcus aureus (Gram-positive bacteria).
- Offers potential against staphylococcal infections (including MRSA).
- Need to understand antimicrobial action mechanisms to develop strategies.
- Antimicrobial inhibitory action can by studied by transcriptome and proteome profiling.
- Generation of protein and mRNA in response to antimicrobial stress are shown in certain cell functions and provide stress type imposed.
- Modeled pathway relationships for 95% of S. aureus MRSA-252 genes by integrating proteome and transcriptome profiling of bacteria that was drug-exposed w/ high-confidence functional association network.
- Approach showed 22 virulence factors and killing mechs. for renalexin along with cell wall effects.
- Evidence of VraRS two-component system in cationic peptide resistance.
- Drug target candidate - FtsH. Role of PhoU-mediated persister formation in S. aureus drug tolerance.
Results and Discussion
Renalexin elicits significant changes in transcript and protein levels
- Sublethal ranalexin impaired but didn't abolish growth of MRSA-252 at concentration of 20μg/ml.
- Proteome and transcriptome profiling applied between control and renalexin exposed MRSA-252 cultures. Microarrays showed:
- 93 upregulated genes.
- 105 downregulated genes.
- No inconsistencies shown
Links
- Alex A. Cardenas
- Week 10 Assignment
- Alex A. Cardenas Week 2
- Alex A. Cardenas Week 3
- Alex A. Cardenas Week 4
- Alex A. Cardenas Week 5
- Alex A. Cardenas Week 6
- Alex A. Cardenas Week 7
- Alex A. Cardenas Week 8
- Alex A. Cardenas Week 9
- Alex A. Cardenas Week 10
- Alex A. Cardenas Week 11
- Alex A. Cardenas Week 12
- Alex A. Cardenas Week 13
- Alex A. Cardenas Week 14
- Class Journal Week 1
- Class Journal Week 2
- Class Journal Week 3
- Class Journal Week 5
- Class Journal Week 6
- Class Journal Week 7
- Class Journal Week 8
- Class Journal Week 9
- Class Journal Week 10
- Class Journal Week 11
- Class Journal Week 12
- Class Journal Week 13
- Class Journal Week 14