Artificial transcriptional terminators

The majority of transcriptional terminators have a G+C rich stem of 7(+/-1)bp and a loop of 4(+/-1) nucleodtides followed by a poly(U) tail. Two common loops are UUCG and GAAA, both of which are known to increase RNA hairpin stability. The sequence GCGGG(G) is a common sequence found on the 3' arm of the stem. [d'Aubenton 90]

• side note*

Several sources [Reynolds 92, Chamberlin 79] measured the termination effiecency(TE) of T7Te at around 90%. However, effiency for the biobricks part BBa_B0012, also T7Te, is around 30%. T7Te has a very short poly(U) tail and requires the further downstream sequence for effiecent termination [Reynolds 92], and this further downstream sequence is lacking in BBa_B0012. If the sequence for BBa_B0012 is lengthened to include this downstream segment, then the TE of part should be improved.

References

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