Artificial transcriptional terminators

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The majority of transcriptional terminators have a G+C rich stem of 7(+/-1)bp and a loop of 4(+/-1) nucleodtides followed by a poly(U) tail. Two common loops are UUCG and GAAA, both of which are known to increase RNA hairpin stability. The sequence GCGGG(G) is a common sequence found on the 3' arm of the stem. [d'Aubenton 90]

  • side note*

Several sources [Reynolds 92, Chamberlin 79] measured the termination effiecency(TE) of T7Te at around 90%. However, effiency for the biobricks part BBa_B0012, also T7Te, is around 30%. T7Te has a very short poly(U) tail and requires the further downstream sequence for effiecent termination [Reynolds 92], and this further downstream sequence is lacking in BBa_B0012. If the sequence for BBa_B0012 is lengthened to include this downstream segment, then the TE of part should be improved.

References

  1. Abe H and Aiba H. . pmid:9150882. PubMed HubMed [Abe96]
  2. d'Aubenton Carafa Y, Brody E, and Thermes C. . pmid:1702475. PubMed HubMed [Aubenton90]
  3. Brendel V, Hamm GH, and Trifonov EN. . pmid:3078109. PubMed HubMed [Bredel86]
  4. Cheng SW, Lynch EC, Leason KR, Court DL, Shapiro BA, and Friedman DI. . pmid:1835546. PubMed HubMed [Cheng91]
  5. Christie GE, Farnham PJ, and Platt T. . pmid:7027254. PubMed HubMed [Christie81]
  6. Ermolaeva MD, Khalak HG, White O, Smith HO, and Salzberg SL. . pmid:10926490. PubMed HubMed [Ermolaeva00]
  7. Lesnik EA, Sampath R, Levene HB, Henderson TJ, McNeil JA, and Ecker DJ. . pmid:11522828. PubMed HubMed [Lesnik01]
  8. Lynn SP, Kasper LM, and Gardner JF. . pmid:2961747. PubMed HubMed [Lynn88]
  9. Petrillo M, Silvestro G, Di Nocera PP, Boccia A, and Paolella G. . pmid:16820051. PubMed HubMed [Petrillo06]
  10. Reynolds R, Bermúdez-Cruz RM, and Chamberlin MJ. . pmid:1372365. PubMed HubMed [Reynolds92I]
  11. Reynolds R and Chamberlin MJ. . pmid:1372366. PubMed HubMed [Reynolds92II]
  12. Unniraman S, Prakash R, and Nagaraja V. . pmid:11809879. PubMed HubMed [Unniraman02]
  13. Uptain SM and Chamberlin MJ. . pmid:9391063. PubMed HubMed [Uptain97]
  14. von Hippel PH and Yager TD. . pmid:1536005. PubMed HubMed [VonHippel92]
  15. Wilson KS and von Hippel PH. . pmid:7966320. PubMed HubMed [Wilson94]
  16. Wilson KS and von Hippel PH. . pmid:7568019. PubMed HubMed [Wilson95]
  17. Yager TD and von Hippel PH. . pmid:1703438. PubMed HubMed [Yager91]
All Medline abstracts: PubMed HubMed
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