Available Vectors

All Vectors

A zip-file containing genbank files of all basic, currently-available part and destination vectors is available here: Media:All_Vectors.zip.

Part Vectors

The pFL and pJT series of part vectors are described in Torella et al. 2014. We recommend using the pJT vectors when assembling into pDestET and pDestBAC destination vectors, and the pFL vectors when assembling into pDestRmceBAC and pDestPBBAC destination vectors. Vector maps are provided in GenBank format below:

Media:pJT_U1U2 (pJT170).gb
Media:pJT_U2U3 (pJT172).gb
Media:pJT_U3U4 (pJT174).gb
Media:pJT_U4U5 (pJT176).gb

Media:pFL_U1U2.gb
Media:pFL_U2U3.gb
Media:pFL_U3U4.gb
Media:pFL_U4U5.gb

Destination Vectors

The pDestET and pDestBAC vectors are used in Torella et al. 2014. The pDestRmceBAC and pDestPBBAC are used in Lienert et al. 2014. The pDestBBR vector is described in a forthcoming manuscript.

Media:pDestET.gb
Media:pDestBAC.gb
Media:pDestRmceBAC.gb
Media:pDestPBBAC.gb
Media:pDestBBR.gb

Obtaining Part and Destination Vectors

Part and Destination vectors can be obtained from the Silver Lab. E-mail jtorella@fas.harvard.edu to request them, or for more information.