Available Vectors: Difference between revisions
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The pDestET and pDestBAC vectors are used in [http://nar.oxfordjournals.org/content/42/1/681.long Torella et al. 2014]. The pDestRmceBAC and pDestPBBAC are used in [http://nar.oxfordjournals.org/content/41/21/9967.long Lienert et al. 2014]. The pDestBBR vector is described in a forthcoming manuscript. | The pDestET and pDestBAC vectors are used in [http://nar.oxfordjournals.org/content/42/1/681.long Torella et al. 2014]. The pDestRmceBAC and pDestPBBAC are used in [http://nar.oxfordjournals.org/content/41/21/9967.long Lienert et al. 2014]. The pDestBBR vector is described in a forthcoming manuscript. | ||
[[Media:pDestET.gb]] (AmpR; 40 copies/cell; ColE1 origin) | [[Media:pDestET.gb]] (AmpR; 40 copies/cell; ColE1 origin; good for strong bacterial expression) | ||
<br>[[Media:pDestBAC.gb]] (CamR 10 ug/mL; 1 copy/cell; BAC origin; amplifiable to 40/cell with arabinose) | <br>[[Media:pDestBAC.gb]] (CamR 10 ug/mL; 1 copy/cell; BAC origin; amplifiable to 40/cell with arabinose; good for stability and decreased leaky gene expression) | ||
<br>[[Media:pDestRmceBAC.gb]] (CamR 10 ug/mL; 1 copy/cell; BAC origin; amplifiable to 40/cell with arabinose) | <br>[[Media:pDestRmceBAC.gb]] (CamR 10 ug/mL; 1 copy/cell; BAC origin; amplifiable to 40/cell with arabinose; good for site-specific integration into mammalian genome) | ||
<br>[[Media:pDestPBBAC.gb]] (CamR 10 ug/mL; 1 copy/cell; BAC origin; amplifiable to 40/cell with arabinose) | <br>[[Media:pDestPBBAC.gb]] (CamR 10 ug/mL; 1 copy/cell; BAC origin; amplifiable to 40/cell with arabinose; good for random integration into mammalian genome) | ||
<br>[[Media:pDestBBR.gb]] (KanR; broad host range BBR origin) | <br>[[Media:pDestBBR.gb]] (KanR; broad host range BBR origin) | ||
Revision as of 11:37, 22 April 2014
All Vectors
A zip-file containing genbank files of all basic, currently-available part and destination vectors is available here: Media:All_Vectors.zip.
Part Vectors
The pFL and pJT series of part vectors are described in Torella et al. 2014. We recommend using the pJT vectors when assembling into pDestET and pDestBAC destination vectors, and the pFL vectors when assembling into pDestRmceBAC and pDestPBBAC destination vectors. Vector maps are provided in GenBank format below:
Media:pJT_U1U2 (pJT170).gb (AmpR; 40 copies/cell)
Media:pJT_U2U3 (pJT172).gb (AmpR; 40 copies/cell)
Media:pJT_U3U4 (pJT174).gb (AmpR; 40 copies/cell)
Media:pJT_U4U5 (pJT176).gb (AmpR; 40 copies/cell)
Media:pFL_U1U2.gb (AmpR; 500 copies/cell)
Media:pFL_U2U3.gb (AmpR; 500 copies/cell)
Media:pFL_U3U4.gb (AmpR; 500 copies/cell)
Media:pFL_U4U5.gb (AmpR; 500 copies/cell)
Destination Vectors
The pDestET and pDestBAC vectors are used in Torella et al. 2014. The pDestRmceBAC and pDestPBBAC are used in Lienert et al. 2014. The pDestBBR vector is described in a forthcoming manuscript.
Media:pDestET.gb (AmpR; 40 copies/cell; ColE1 origin; good for strong bacterial expression)
Media:pDestBAC.gb (CamR 10 ug/mL; 1 copy/cell; BAC origin; amplifiable to 40/cell with arabinose; good for stability and decreased leaky gene expression)
Media:pDestRmceBAC.gb (CamR 10 ug/mL; 1 copy/cell; BAC origin; amplifiable to 40/cell with arabinose; good for site-specific integration into mammalian genome)
Media:pDestPBBAC.gb (CamR 10 ug/mL; 1 copy/cell; BAC origin; amplifiable to 40/cell with arabinose; good for random integration into mammalian genome)
Media:pDestBBR.gb (KanR; broad host range BBR origin)
Obtaining Part and Destination Vectors
Part and Destination vectors can be obtained from the Silver Lab. E-mail jtorella@fas.harvard.edu to request them, or for more information.
