Axel:Schwekendiek Tutorials: Difference between revisions
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This page has been "stolen" from [http://openwetware.org/wiki/Arking:JCAOligoTutorialHome Prof. John C. Anderson at UC Berkeley]. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. And until I am done, I hope John will keep his valuable pages online. | This page has been "stolen" from [http://openwetware.org/wiki/Arking:JCAOligoTutorialHome Prof. John C. Anderson at UC Berkeley]. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. And until I am done, I hope John will keep his valuable pages online and the links working. If the links are dead, please let me know and will hurry replacing them. | ||
==The Basics== | ==The Basics== | ||
Revision as of 14:46, 18 August 2009
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Schwekendiek Lab |
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This page has been "stolen" from Prof. John C. Anderson at UC Berkeley. I could as well just have linked to it, but I decided to copy it because I want to replace the content with my own. Since I know me, this will take forever. And until I am done, I hope John will keep his valuable pages online and the links working. If the links are dead, please let me know and will hurry replacing them.
The Basics
Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics.
Bare bones biochemistry
Basic Molecular Biology
Practical DNA
What is PCR?
Introduction to Biobricks and Basic Cloning
This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it.
Installing ApE
Overview of Cloning
Introduction to Biobricks
Introduction to Refactoring
Technical Design
Basic Oligo Design
Special Case: Single Internal Restriction Sites
Construction of short parts
Design of composite parts
Keeping track of construction files
Sequencing Analysis
Advanced Technical Design
Working with Translational Fusions
Circularly Permuted Proteins
The "what did they make" game
Implementation
Implementing the Basic Construction File
Introduction to Structural Biology and Part Engineering
DeepView Basics
Tour of Structural Biology
Things you should know
Common Pitfalls and Tips
Origins of replication
The Critical Path
