BCH070505-Construction of pJ23006-J23007: Difference between revisions
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[EcoRI][TT][Ptet][XbaI][Ptet][RFP][TT][SpeI][PstI]+[XbaI][Key3b][SpeI][PstI]<br> | |||
[EcoRI][TT][Ptet][XbaI][Ptet][RFP][TT][SpeI][PstI]+[XbaI][Key3b][SpeI][PstI] | Digest J23007 (XbaI/PstI, Neb2, ~55bp, use small frag cleanup)<br> | ||
Digest J23007 (XbaI/PstI, Neb2, ~55bp, use small frag cleanup) | Paste into pJ23006 (XbaI/PstI, Neb2, Large-2334bp)<br> | ||
Paste into pJ23006 (XbaI/PstI, Neb2, Large-2334bp) | |||
Product is pJ23006-J23007 | Product is pJ23006-J23007 | ||
Revision as of 11:03, 5 July 2006
[EcoRI][TT][Ptet][XbaI][Ptet][RFP][TT][SpeI][PstI]+[XbaI][Key3b][SpeI][PstI]
Digest J23007 (XbaI/PstI, Neb2, ~55bp, use small frag cleanup)
Paste into pJ23006 (XbaI/PstI, Neb2, Large-2334bp)
Product is pJ23006-J23007
