BCH081006-Construction of pJ23006-J23062 key/tRNA
From OpenWetWare
PCR ca998/G00101 on J23009variant.........(478bp, BamHI/HindIII, small sequence workup)
Sub into pJ23006-J23061...................(BamHI/HindIII)
Product is pJ23006-J23062, screen for loss of XhoI