BCH4160/2011:Notebook/Laura Lee/2011/09/20: Difference between revisions

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==Materials==
==Materials==
List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.


*supply 1 (i.e. tubes of a certain size? spreaders?)
Materials below provide by Dr. J. Cannon.
*reagent 1
 
*X μL reagent 2
*CHCl3
**component A (reagent 2 is made up of multiple components)
*MeOH
**component B
*Lipid solution (?)
*equipment 1
*Vial
*equipment 2


==Procedure==
==Procedure==

Revision as of 08:05, 16 November 2011

Preparation of Lipid Films

The purpose of this protocol was to prepare lipid films for use in future experiments.

Materials

Materials below provide by Dr. J. Cannon.

  • CHCl3
  • MeOH
  • Lipid solution (?)
  • Vial

Procedure

  1. Step 1
  2. Step 2
    • Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
  3. Step 3
    1. Step 3 has multiple sub-steps within it.
    2. Enumerate each of those.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. Goldbeter, A and Koshland, DE (1981) - Proc Natl Acad Sci U S A 78(11) 6840-4 PMID 6947258
  2. Jacob, F and Monod, J J (1961) - Mol Biol 3(3) 318-56 PMID 13718526
  3. Ptashne, M (2004) Genetic Switch: Phage Lambda Revisited - Cold Spring Harbor Laboratory Press ISBN 0879697164

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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