BCH4160/2011:Notebook/Laura Lee/2011/09/20: Difference between revisions
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==Materials== | ==Materials== | ||
Materials below | Materials below provided by Dr. J. Cannon. | ||
*CHCl3 | *CHCl3 |
Revision as of 08:05, 16 November 2011
Preparation of Lipid Films
The purpose of this protocol was to prepare lipid films for use in future experiments.
Materials
Materials below provided by Dr. J. Cannon.
- CHCl3
- MeOH
- Lipid solution (?)
- Vial
Procedure
- Step 1
- Step 2
- Step 2 has some additional information that goes with it. i.e. Keep at 4°C.
- Step 3
- Step 3 has multiple sub-steps within it.
- Enumerate each of those.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.
References
Relevant papers and books
- Goldbeter, A and Koshland, DE (1981) - Proc Natl Acad Sci U S A 78(11) 6840-4 PMID 6947258
- Jacob, F and Monod, J J (1961) - Mol Biol 3(3) 318-56 PMID 13718526
- Ptashne, M (2004) Genetic Switch: Phage Lambda Revisited - Cold Spring Harbor Laboratory Press ISBN 0879697164
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.