BCH4160/2011:Notebook/Laura Lee/2011/09/20: Difference between revisions

Revision as of 08:10, 16 November 2011

The purpose of this protocol was to prepare lipid films for use in future experiments.

Materials below provided by Dr. J. Cannon.

Add 6mL CHCl3 and 2mL MeOH for a total of 8mL of 3:1 CHCl3:MeOH.
Take 2.5mL of CHCl3/MeOH solution and add it to the lipid solution in the vial (will have 2 vials total).
Use 1mL of lipid/CHCl3/MeOH solution and place it in a 4mL vial (should have 5 vials total).
Dry the vials using a roto-evaporator.
Place vial in a desicator overnight to thoroughly dry.
Store vials in freeze for future use.

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

List troubleshooting tips here.
You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

Relevant papers and books

Goldbeter, A and Koshland, DE (1981) - Proc Natl Acad Sci U S A 78(11) 6840-4 PMID 6947258
Jacob, F and Monod, J J (1961) - Mol Biol 3(3) 318-56 PMID 13718526
Ptashne, M (2004) Genetic Switch: Phage Lambda Revisited - Cold Spring Harbor Laboratory Press ISBN 0879697164

@@ Line 13: / Line 13: @@
 ==Procedure==
-#Step 1
-#Step 2
+#Add 6mL CHCl3 and 2mL MeOH for a total of 8mL of 3:1 CHCl3:MeOH.
-#*Step 2 has some additional information that goes with it.  i.e. Keep at 4&deg;C.
+#Take 2.5mL of CHCl3/MeOH solution and add it to the lipid solution in the vial (will have 2 vials total).
-#Step 3
+#Use 1mL of lipid/CHCl3/MeOH solution and place it in a 4mL vial (should have 5 vials total).
-##Step 3 has multiple sub-steps within it.
+#Dry the vials using a roto-evaporator.
-##Enumerate each of those.
+#Place vial in a desicator overnight to thoroughly dry.
+#Store vials in freeze for future use.
 ==Notes==