BCH4160/2011:Notebook/Laura Lee/2011/09/20: Difference between revisions
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#Place vial in a desicator overnight to thoroughly dry. | #Place vial in a desicator overnight to thoroughly dry. | ||
#Store vials in freeze for future use. | #Store vials in freeze for future use. | ||
Revision as of 08:11, 16 November 2011
Preparation of Lipid Films
The purpose of this protocol was to prepare lipid films for use in future experiments.
Materials
Materials below provided by Dr. J. Cannon.
- CHCl3
- MeOH
- Lipid solution (?)
- Vial
Procedure
- Add 6mL CHCl3 and 2mL MeOH for a total of 8mL of 3:1 CHCl3:MeOH.
- Take 2.5mL of CHCl3/MeOH solution and add it to the lipid solution in the vial (will have 2 vials total).
- Use 1mL of lipid/CHCl3/MeOH solution and place it in a 4mL vial (should have 5 vials total).
- Dry the vials using a roto-evaporator.
- Place vial in a desicator overnight to thoroughly dry.
- Store vials in freeze for future use.