BCH4160/2011:Notebook/Laura Lee/2011/09/20

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Preparation of Lipid Films

The purpose of this protocol was to prepare lipid films for use in future experiments.

Materials

Materials below provided by Dr. J. Cannon.

  • CHCl3
  • MeOH
  • Lipid solution (?)
  • Vial

Procedure

  1. Add 6mL CHCl3 and 2mL MeOH for a total of 8mL of 3:1 CHCl3:MeOH.
  2. Take 2.5mL of CHCl3/MeOH solution and add it to the lipid solution in the vial (will have 2 vials total).
  3. Use 1mL of lipid/CHCl3/MeOH solution and place it in a 4mL vial (should have 5 vials total).
  4. Dry the vials using a roto-evaporator.
  5. Place vial in a desicator overnight to thoroughly dry.
  6. Store vials in freeze for future use.


References

Relevant papers and books

  1. Goldbeter, A and Koshland, DE (1981) - Proc Natl Acad Sci U S A 78(11) 6840-4 PMID 6947258
  2. Jacob, F and Monod, J J (1961) - Mol Biol 3(3) 318-56 PMID 13718526
  3. Ptashne, M (2004) Genetic Switch: Phage Lambda Revisited - Cold Spring Harbor Laboratory Press ISBN 0879697164

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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