BCH4160/2011:Notebook/Laura Lee/2011/10/11
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PCR continued
Refer to part 1 for purpose of experiment.
Materials
Refer to part 1 for initial materials. Refer to "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc. for complete list of materials.
Gel electrophoresis/Staining of Gel
- Gel electrophoresis system
- Previously amplified DNA samples (from part 1)
- Agarose
- 50x TAE buffer
- Fast Blast™ DNA Stain
Procedure
The purpose of this part of the procedure was to use gel electrophoresis to separate and visualize the DNA fragments.
Refer to "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc. for the complete procedure.
Results
No results were gained from this experiments. Errors were made in gel staining (overstaining) and bands were not visually distinguishable.
Contact
This experiment was done with High Point University students Jigesh Patel and Brittany Webster.
