BE.109:Bio-material engineering
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In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction. | In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction. | ||
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| + | [[Image:Macintosh HD-Users-nkuldell-Desktop-yeastbuddingandbound.png|center|400px|'''S. cerevisiae shown budding (top) and bound to CdS (bottom)''']] | ||
==Lab handouts== | ==Lab handouts== | ||
Revision as of 23:58, 2 January 2006
Module 4
Instructors: Angela Belcher and Natalie Kuldell
TA: Eileen Higham
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
Lab handouts
Day 2: Panning for gold binders
Day 3: Screening library and optimizing panning
