BE.109:Bio-material engineering

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'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate.
'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate.
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#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum| URL] [[Image:Macintosh HD-Users-nkuldell-Desktop-BE109-BE109 lab manual S06-Mod4 (S06)-MetalBindingYeast Lang05.pdf|PDF reprint]] [[Image:Macintosh HD-Users-nkuldell-Desktop-BE109-BE109 lab manual S06-Mod4 (S06)-MetalBindYeast Sup1 Lang05.pdf | first supplement to pdf]] [[Image:Macintosh HD-Users-nkuldell-Desktop-BE109-BE109 lab manual S06-Mod4 (S06)-MetalBindYeast Sup2 Lang05.pdf | second supplement to pdf]]
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#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum| URL] [http://openwetware.org/images/e/ee/Macintosh_HD-Users-nkuldell-Desktop-BE109-BE109_lab_manual_S06-Mod4_%28S06%29-MetalBindingYeast_Lang05.pdf PDF reprint] [http://openwetware.org/images/f/fa/Macintosh_HD-Users-nkuldell-Desktop-BE109-BE109_lab_manual_S06-Mod4_%28S06%29-MetalBindYeast_Sup1_Lang05.pdf Supplementary figures] [http://openwetware.org/images/a/a3/Macintosh_HD-Users-nkuldell-Desktop-BE109-BE109_lab_manual_S06-Mod4_%28S06%29-MetalBindYeast_Sup2_Lang05.pdf Supplementary table]
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Revision as of 19:23, 26 February 2006

BE.109 Laboratory Fundamentals of Biological Engineering

Image:BE LongImage-1.jpg

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DNA engineering        Protein engineering        Systems engineering        Bio-material engineering       


Module 4

Instructors: Angela Belcher and Natalie Kuldell

TA: Eileen Higham

In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.

S. cerevisiae shown budding (top) and bound to CdS (bottom)

Lab handouts

Day 1: Screening library

Day 2: Optimizing panning

Day 3: Rescreening gold binders

Day 4: PCR of gold binding candidates

Day 5: Student presentations

Day 6: Sequence analysis

References

Note: PDF reprints are provided below within the context of fair use. Please obtain copies from the publisher if appropriate.

  1. Design criteria for engineering inorganic material-specific peptides.
    Langmuir 2005 21(15):6929-6933
    Peelle BR, Krauland EM, Wittrup KD, Belcher AM
    URL PDF reprint Supplementary figures Supplementary table


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