BE.109:Bio-material engineering

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'''Instructors:''' Angela Belcher and [[Natalie Kuldell]]
'''Instructors:''' Angela Belcher and [[Natalie Kuldell]]
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'''TA:''' Eileen Higham
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'''TA:''' [[Eileen Higham]]
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
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[[Image:Macintosh HD-Users-nkuldell-Desktop-yeastbuddingandbound.png|center|400px|'''S. cerevisiae shown budding (top) and bound to CdS (bottom)''']]
==Lab handouts==
==Lab handouts==
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'''[[BE.109:Bio-material engineering/Getting to know yeast | Day 1: Getting to know yeast]]'''
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'''[[BE.109:Bio-material engineering/Screening library | Day 1: Screening library]]'''
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'''[[BE.109:Bio-material engineering/Panning for gold binders | Day 2: Panning for gold binders]]'''
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'''[[BE.109:Bio-material engineering/Optimizing panning | Day 2: Optimizing panning]]'''
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'''[[BE.109:Bio-material engineering/Screening library and optimizing panning | Day 3: Screening library and optimizing panning]]'''
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'''[[BE.109:Bio-material engineering/Rescreening gold binders | Day 3: Rescreening gold binders]]'''
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'''[[BE.109:Bio-material engineering/Checking gold binders | Day 4: Checking gold binders]]'''
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'''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 4: PCR of gold binding candidates]]'''
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'''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 5: PCR of gold binding candidates]]'''
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'''[[BE.109:Presenting your work | Day 5: Student presentations]]'''
'''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]'''
'''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]'''
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==References==
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'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate.
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#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum| URL]
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Current revision

BE.109 Laboratory Fundamentals of Biological Engineering

Image:BE LongImage-1.jpg

Home        Getting started        Lab        Presenting your work        People        Schedule       

DNA engineering        Protein engineering        Systems engineering        Bio-material engineering       


Module 4

Instructors: Angela Belcher and Natalie Kuldell

TA: Eileen Higham

In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.

S. cerevisiae shown budding (top) and bound to CdS (bottom)

Lab handouts

Day 1: Screening library

Day 2: Optimizing panning

Day 3: Rescreening gold binders

Day 4: PCR of gold binding candidates

Day 5: Student presentations

Day 6: Sequence analysis

References

Note: PDF reprints are provided below within the context of fair use. Please obtain copies from the publisher if appropriate.

  1. Design criteria for engineering inorganic material-specific peptides.
    Langmuir 2005 21(15):6929-6933
    Peelle BR, Krauland EM, Wittrup KD, Belcher AM
    URL


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