BE.109:Bio-material engineering

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==Module 4==
==Module 4==
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
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==Lab handouts==
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'''[[BE.109:Bio-material engineering day 1 | Day 1]]'''
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'''[[BE.109:Bio-material engineering day 2 | Day 2]]'''
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'''[[BE.109:Bio-material engineering day 3 | Day 3]]'''
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'''[[BE.109:Bio-material engineering day 4 | Day 4]]'''
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'''[[BE.109:Bio-material engineering day 5 | Day 5]]'''

Revision as of 18:50, 20 November 2005

BE.109 Laboratory Fundamentals of Biological Engineering

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DNA engineering        Protein engineering        Systems engineering        Bio-material engineering       


Module 4

In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.

Lab handouts

Day 1

Day 2

Day 3

Day 4

Day 5

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