BE.109:Bio-material engineering

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Lab handouts)
(Lab handouts)
Line 9: Line 9:
'''[[BE.109:Bio-material engineering/Getting to know yeast | Day 1]]'''
'''[[BE.109:Bio-material engineering/Getting to know yeast | Day 1]]'''
-
'''[[BE.109:Bio-material engineering day 2 | Day 2]]'''
+
'''[[BE.109:Bio-material engineering/Panning for gold binders | Day 2]]'''
-
'''[[BE.109:Bio-material engineering day 3 | Day 3]]'''
+
'''[[BE.109:Bio-material engineering/Screening library and optimizing panning | Day 3]]'''
-
'''[[BE.109:Bio-material engineering day 4 | Day 4]]'''
+
'''[[BE.109:Bio-material engineering/Checking gold binders | Day 4]]'''
-
'''[[BE.109:Bio-material engineering day 5 | Day 5]]'''
+
'''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 5]]'''
 +
 
 +
'''[[BE.109:Bio-material engineering/Sequence analysis | Day 6]]'''

Revision as of 20:11, 20 November 2005

BE.109 Laboratory Fundamentals of Biological Engineering

Image:BE LongImage-1.jpg

Home        Getting started        Lab        Presenting your work        People        Schedule       

DNA engineering        Protein engineering        Systems engineering        Bio-material engineering       


Module 4

In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.

Lab handouts

Day 1

Day 2

Day 3

Day 4

Day 5

Day 6

Personal tools