BE.109:Bio-material engineering: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 9: | Line 9: | ||
'''[[BE.109:Bio-material engineering/Getting to know yeast | Day 1]]''' | '''[[BE.109:Bio-material engineering/Getting to know yeast | Day 1]]''' | ||
'''[[BE.109:Bio-material engineering | '''[[BE.109:Bio-material engineering/Panning for gold binders | Day 2]]''' | ||
'''[[BE.109:Bio-material engineering | '''[[BE.109:Bio-material engineering/Screening library and optimizing panning | Day 3]]''' | ||
'''[[BE.109:Bio-material engineering | '''[[BE.109:Bio-material engineering/Checking gold binders | Day 4]]''' | ||
'''[[BE.109:Bio-material engineering | '''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 5]]''' | ||
'''[[BE.109:Bio-material engineering/Sequence analysis | Day 6]]''' | |||
Revision as of 17:11, 20 November 2005
Module 4
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
