BE.109:Bio-material engineering
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==Lab handouts== | ==Lab handouts== | ||
| - | '''[[BE.109:Bio-material engineering/Getting to know yeast | Day 1]]''' | + | '''[[BE.109:Bio-material engineering/Getting to know yeast | Day 1: Getting to know yeast]]''' |
| - | '''[[BE.109:Bio-material engineering/Panning for gold binders | Day 2]]''' | + | '''[[BE.109:Bio-material engineering/Panning for gold binders | Day 2: Panning for gold binders]]''' |
| - | '''[[BE.109:Bio-material engineering/Screening library and optimizing panning | Day 3]]''' | + | '''[[BE.109:Bio-material engineering/Screening library and optimizing panning | Day 3: Screening library and optimizing panning]]''' |
| - | '''[[BE.109:Bio-material engineering/Checking gold binders | Day 4]]''' | + | '''[[BE.109:Bio-material engineering/Checking gold binders | Day 4: Checking gold binders]]''' |
| - | '''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 5]]''' | + | '''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 5: PCR of gold binding candidates]]''' |
| - | '''[[BE.109:Bio-material engineering/Sequence analysis | Day 6]]''' | + | '''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]''' |
Revision as of 19:54, 27 November 2005
Module 4
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
Lab handouts
Day 2: Panning for gold binders
Day 3: Screening library and optimizing panning
