BE.109:Bio-material engineering

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(Lab handouts)
(Lab handouts)
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==Lab handouts==
==Lab handouts==
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'''[[BE.109:Bio-material engineering/Getting to know yeast | Day 1]]'''
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'''[[BE.109:Bio-material engineering/Getting to know yeast | Day 1: Getting to know yeast]]'''
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'''[[BE.109:Bio-material engineering/Panning for gold binders | Day 2]]'''
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'''[[BE.109:Bio-material engineering/Panning for gold binders | Day 2: Panning for gold binders]]'''
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'''[[BE.109:Bio-material engineering/Screening library and optimizing panning | Day 3]]'''
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'''[[BE.109:Bio-material engineering/Screening library and optimizing panning | Day 3: Screening library and optimizing panning]]'''
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'''[[BE.109:Bio-material engineering/Checking gold binders | Day 4]]'''
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'''[[BE.109:Bio-material engineering/Checking gold binders | Day 4: Checking gold binders]]'''
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'''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 5]]'''
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'''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 5: PCR of gold binding candidates]]'''
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'''[[BE.109:Bio-material engineering/Sequence analysis | Day 6]]'''
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'''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]'''

Revision as of 18:54, 27 November 2005

BE.109 Laboratory Fundamentals of Biological Engineering

Image:BE LongImage-1.jpg

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DNA engineering        Protein engineering        Systems engineering        Bio-material engineering       


Module 4

In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.

Lab handouts

Day 1: Getting to know yeast

Day 2: Panning for gold binders

Day 3: Screening library and optimizing panning

Day 4: Checking gold binders

Day 5: PCR of gold binding candidates

Day 6: Sequence analysis

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