BE.109:Bio-material engineering: Difference between revisions
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==Module 4== | ==Module 4== | ||
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'''Instructors:''' Angela Belcher and [[Natalie Kuldell]] | '''Instructors:''' Angela Belcher and [[Natalie Kuldell]] | ||
'''TA:''' Eileen Higham | '''TA:''' [[Eileen Higham]] | ||
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction. | In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction. | ||
[[Image:Macintosh HD-Users-nkuldell-Desktop-yeastbuddingandbound.png|center|400px|'''S. cerevisiae shown budding (top) and bound to CdS (bottom)''']] | |||
==Lab handouts== | ==Lab handouts== | ||
'''[[BE.109:Bio-material engineering/ | '''[[BE.109:Bio-material engineering/Screening library | Day 1: Screening library]]''' | ||
'''[[BE.109:Bio-material engineering/ | '''[[BE.109:Bio-material engineering/Optimizing panning | Day 2: Optimizing panning]]''' | ||
'''[[BE.109:Bio-material engineering/ | '''[[BE.109:Bio-material engineering/Rescreening gold binders | Day 3: Rescreening gold binders]]''' | ||
'''[[BE.109:Bio-material engineering/ | '''[[BE.109:Bio-material engineering/PCR of gold binding candidates | Day 4: PCR of gold binding candidates]]''' | ||
'''[[BE.109: | '''[[BE.109:Presenting your work | Day 5: Student presentations]]''' | ||
'''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]''' | '''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]''' | ||
==References== | |||
'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate. | |||
#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum| URL] | |||
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Latest revision as of 12:42, 9 October 2006
Module 4
Instructors: Angela Belcher and Natalie Kuldell
TA: Eileen Higham
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
Lab handouts
Day 3: Rescreening gold binders
Day 4: PCR of gold binding candidates
References
Note: PDF reprints are provided below within the context of fair use. Please obtain copies from the publisher if appropriate.
- Design criteria for engineering inorganic material-specific peptides.
Langmuir 2005 21(15):6929-6933
Peelle BR, Krauland EM, Wittrup KD, Belcher AM
URL
