BE.109:Bio-material engineering: Difference between revisions
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'''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]''' | '''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]''' | ||
==References== | |||
'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate. | |||
#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum| URL] [[Image:Macintosh HD-Users-nkuldell-Desktop-BE109-BE109 lab manual S06-Mod4 (S06)-MetalBindingYeast Lang05.pdf|PDF reprint]] [[Image:Macintosh HD-Users-nkuldell-Desktop-BE109-BE109 lab manual S06-Mod4 (S06)-MetalBindYeast Sup1 Lang05.pdf | first supplement to pdf]] [[Image:Macintosh HD-Users-nkuldell-Desktop-BE109-BE109 lab manual S06-Mod4 (S06)-MetalBindYeast Sup2 Lang05.pdf | second supplement to pdf]] | |||
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Revision as of 04:06, 9 January 2006
Module 4
Instructors: Angela Belcher and Natalie Kuldell
TA: Eileen Higham
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
Lab handouts
Day 2: Panning for gold binders
Day 3: Screening library and optimizing panning
Day 5: PCR of gold binding candidates
References
Note: PDF reprints are provided below within the context of fair use. Please obtain copies from the publisher if appropriate.
- Design criteria for engineering inorganic material-specific peptides.
Langmuir 2005 21(15):6929-6933
Peelle BR, Krauland EM, Wittrup KD, Belcher AM
URL PDF reprint first supplement to pdf second supplement to pdf
