BE.109:Bio-material engineering: Difference between revisions
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'''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]''' | '''[[BE.109:Bio-material engineering/Sequence analysis | Day 6: Sequence analysis]]''' | ||
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Revision as of 10:14, 5 December 2005
Module 4
Instructors: Angela Belcher and Natalie Kuldell
TA: Eileen Higham
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
Lab handouts
Day 2: Panning for gold binders
Day 3: Screening library and optimizing panning
