BE.109:Bio-material engineering - Revision history

Reshma P. Shetty: /* References */

Reshma P. Shetty — Mon, 09 Oct 2006 19:42:44 GMT

References

←Older revision		Revision as of 19:42, 9 October 2006
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	'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate.		'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate.

-	#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum\| URL] [http://openwetware.org/images/e/ee/Macintosh_HD-Users-nkuldell-Desktop-BE109-BE109_lab_manual_S06-Mod4_%28S06%29-MetalBindingYeast_Lang05.pdf PDF reprint] [http://openwetware.org/images/f/fa/Macintosh_HD-Users-nkuldell-Desktop-BE109-BE109_lab_manual_S06-Mod4_%28S06%29-MetalBindYeast_Sup1_Lang05.pdf Supplementary figures] [http://openwetware.org/images/a/a3/Macintosh_HD-Users-nkuldell-Desktop-BE109-BE109_lab_manual_S06-Mod4_%28S06%29-MetalBindYeast_Sup2_Lang05.pdf Supplementary table]	+	#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum\| URL]


	</div>		</div>

Reshma P. Shetty: /* References */

Reshma P. Shetty — Mon, 27 Feb 2006 00:23:30 GMT

References

←Older revision		Revision as of 00:23, 27 February 2006
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	'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate.		'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate.

-	#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum\| URL] [~~[Image~~:~~Macintosh HD~~-Users-nkuldell-Desktop-BE109-~~BE109 lab manual S06~~-~~Mod4 (S06)~~-~~MetalBindingYeast Lang05~~.pdf\|PDF reprint]~~] [~~[~~Image~~:~~Macintosh HD~~-Users-nkuldell-Desktop-BE109-~~BE109 lab manual S06~~-~~Mod4 (S06)~~-~~MetalBindYeast Sup1 Lang05~~.pdf ~~\| first supplement to pdf]~~] [~~[Image~~:~~Macintosh HD~~-Users-nkuldell-Desktop-BE109-~~BE109 lab manual S06~~-~~Mod4 (S06)~~-~~MetalBindYeast Sup2 Lang05~~.pdf ~~\| second supplement to pdf]~~]	+	#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum\| URL] [http://openwetware.org/images/e/ee/Macintosh_HD-Users-nkuldell-Desktop-BE109-BE109_lab_manual_S06-Mod4_%28S06%29-MetalBindingYeast_Lang05.pdf PDF reprint] [http://openwetware.org/images/f/fa/Macintosh_HD-Users-nkuldell-Desktop-BE109-BE109_lab_manual_S06-Mod4_%28S06%29-MetalBindYeast_Sup1_Lang05.pdf Supplementary figures] [http://openwetware.org/images/a/a3/Macintosh_HD-Users-nkuldell-Desktop-BE109-BE109_lab_manual_S06-Mod4_%28S06%29-MetalBindYeast_Sup2_Lang05.pdf Supplementary table]


	</div>		</div>

Reshma P. Shetty: /* Lab handouts */ name changes

Reshma P. Shetty — Mon, 06 Feb 2006 02:56:34 GMT

Lab handouts: name changes

←Older revision		Revision as of 02:56, 6 February 2006
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	==Lab handouts==		==Lab handouts==

-	'''[[BE.109:Bio-material engineering/~~Panning for gold binders~~ \| Day 1: Screening library]]'''	+	'''[[BE.109:Bio-material engineering/Screening library \| Day 1: Screening library]]'''

-	'''[[BE.109:Bio-material engineering/~~Getting to know yeast~~ \| Day 2: Optimizing panning]]'''	+	'''[[BE.109:Bio-material engineering/Optimizing panning \| Day 2: Optimizing panning]]'''

-	'''[[BE.109:Bio-material engineering/~~Checking~~ gold binders \| Day 3: Rescreening gold binders]]'''	+	'''[[BE.109:Bio-material engineering/Rescreening gold binders \| Day 3: Rescreening gold binders]]'''

	'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''		'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''

Reshma P. Shetty: /* Lab handouts */ minor formatting fix

Reshma P. Shetty — Wed, 25 Jan 2006 16:00:56 GMT

Lab handouts: minor formatting fix

←Older revision		Revision as of 16:00, 25 January 2006
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	'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''		'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''

-	'''[[~~http://openwetware.org/wiki/~~BE.109~~%3APresenting_your_work~~ \| Day 5: Student presentations]]'''	+	'''[[BE.109:Presenting your work \| Day 5: Student presentations]]'''

	'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''		'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''

Nkuldell: /* Lab handouts */

Nkuldell — Wed, 25 Jan 2006 15:33:20 GMT

Lab handouts

←Older revision		Revision as of 15:33, 25 January 2006
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	==Lab handouts==		==Lab handouts==

-	'''[[BE.109:Bio-material engineering/~~Getting to know yeast~~ \| Day 1: Screening library]]'''	+	'''[[BE.109:Bio-material engineering/Panning for gold binders \| Day 1: Screening library]]'''

-	'''[[BE.109:Bio-material engineering/~~Panning for gold binders~~ \| Day 2: Optimizing panning]]'''	+	'''[[BE.109:Bio-material engineering/Getting to know yeast \| Day 2: Optimizing panning]]'''

	'''[[BE.109:Bio-material engineering/Checking gold binders \| Day 3: Rescreening gold binders]]'''		'''[[BE.109:Bio-material engineering/Checking gold binders \| Day 3: Rescreening gold binders]]'''

Nkuldell: /* Lab handouts */

Nkuldell — Wed, 25 Jan 2006 15:26:39 GMT

Lab handouts

←Older revision		Revision as of 15:26, 25 January 2006
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	==Lab handouts==		==Lab handouts==

-	'''[[BE.109:Bio-material engineering/Getting to know yeast \| Day 1: ~~Getting to know yeast~~]]'''	+	'''[[BE.109:Bio-material engineering/Getting to know yeast \| Day 1: Screening library]]'''

-	'''[[BE.109:Bio-material engineering/Panning for gold binders \| Day 2: ~~Panning for gold binders~~]]'''	+	'''[[BE.109:Bio-material engineering/Panning for gold binders \| Day 2: Optimizing panning]]'''

-	'''[[BE.109:Bio-material engineering/~~Screening library and optimizing panning~~ \| Day 3: ~~Screening library and optimizing panning~~]]'''	+	'''[[BE.109:Bio-material engineering/Checking gold binders \| Day 3: Rescreening gold binders]]'''

-	'''[[BE.109:Bio-material engineering/~~Checking~~ gold ~~binders~~ \| Day 4: ~~Checking~~ gold ~~binders~~]]'''	+	'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''

-	'''[[BE.109~~:Bio-material engineering/PCR of gold binding candidates~~ \| Day 5: ~~PCR of gold binding candidates~~]]'''	+	'''[[http://openwetware.org/wiki/BE.109%3APresenting_your_work \| Day 5: Student presentations]]'''

	'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''		'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''

Emhigham: /* Module 4 */

Emhigham — Wed, 11 Jan 2006 16:00:36 GMT

Module 4

←Older revision		Revision as of 16:00, 11 January 2006
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	'''Instructors:''' Angela Belcher and [[Natalie Kuldell]]		'''Instructors:''' Angela Belcher and [[Natalie Kuldell]]

-	'''TA:''' Eileen Higham	+	'''TA:''' [[Eileen Higham]]

	In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.		In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.

Nkuldell at 11:06, 9 January 2006

Nkuldell — Mon, 09 Jan 2006 11:06:43 GMT

←Older revision		Revision as of 11:06, 9 January 2006
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	'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''		'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''
		+
		+	==References==
		+
		+	'''Note:''' PDF reprints are provided below within the context of [http://www.copyright.gov/fls/fl102.html fair use]. Please obtain copies from the publisher if appropriate.
		+
		+	#'''Design criteria for engineering inorganic material-specific peptides.'''<br>''Langmuir'' 2005 '''21(15)''':6929-6933 <br> Peelle BR, Krauland EM, Wittrup KD, Belcher AM<br> [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16008405&query_hl=4&itool=pubmed_docsum\| URL] [[Image:Macintosh HD-Users-nkuldell-Desktop-BE109-BE109 lab manual S06-Mod4 (S06)-MetalBindingYeast Lang05.pdf\|PDF reprint]] [[Image:Macintosh HD-Users-nkuldell-Desktop-BE109-BE109 lab manual S06-Mod4 (S06)-MetalBindYeast Sup1 Lang05.pdf \| first supplement to pdf]] [[Image:Macintosh HD-Users-nkuldell-Desktop-BE109-BE109 lab manual S06-Mod4 (S06)-MetalBindYeast Sup2 Lang05.pdf \| second supplement to pdf]]
		+

	</div>		</div>

Nkuldell: /* Module 4 */

Nkuldell — Tue, 03 Jan 2006 03:58:33 GMT

Module 4

←Older revision		Revision as of 03:58, 3 January 2006
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	In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.		In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
		+
		+	[[Image:Macintosh HD-Users-nkuldell-Desktop-yeastbuddingandbound.png\|center\|400px\|'''S. cerevisiae shown budding (top) and bound to CdS (bottom)''']]

	==Lab handouts==		==Lab handouts==

Reshma P. Shetty at 17:14, 5 December 2005

Reshma P. Shetty — Mon, 05 Dec 2005 17:14:28 GMT

←Older revision		Revision as of 17:14, 5 December 2005
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	{{Template:BE.109}}		{{Template:BE.109}}

		+
		+	<div style="padding: 10px; width: 720px; border: 5px solid #2D9258;">

	==Module 4==		==Module 4==
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	'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''		'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''
		+
		+	</div>

←Older revision		Revision as of 02:56, 6 February 2006
Line 16:		Line 16:
	==Lab handouts==		==Lab handouts==

-	'''[[BE.109:Bio-material engineering/~~Panning for gold binders~~ \| Day 1: Screening library]]'''	+	'''[[BE.109:Bio-material engineering/Screening library \| Day 1: Screening library]]'''

-	'''[[BE.109:Bio-material engineering/~~Getting to know yeast~~ \| Day 2: Optimizing panning]]'''	+	'''[[BE.109:Bio-material engineering/Optimizing panning \| Day 2: Optimizing panning]]'''

-	'''[[BE.109:Bio-material engineering/~~Checking~~ gold binders \| Day 3: Rescreening gold binders]]'''	+	'''[[BE.109:Bio-material engineering/Rescreening gold binders \| Day 3: Rescreening gold binders]]'''

	'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''		'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''

←Older revision		Revision as of 16:00, 25 January 2006
Line 24:		Line 24:
	'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''		'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''

-	'''[[~~http://openwetware.org/wiki/~~BE.109~~%3APresenting_your_work~~ \| Day 5: Student presentations]]'''	+	'''[[BE.109:Presenting your work \| Day 5: Student presentations]]'''

	'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''		'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''

←Older revision		Revision as of 15:26, 25 January 2006
Line 16:		Line 16:
	==Lab handouts==		==Lab handouts==

-	'''[[BE.109:Bio-material engineering/Getting to know yeast \| Day 1: ~~Getting to know yeast~~]]'''	+	'''[[BE.109:Bio-material engineering/Getting to know yeast \| Day 1: Screening library]]'''

-	'''[[BE.109:Bio-material engineering/Panning for gold binders \| Day 2: ~~Panning for gold binders~~]]'''	+	'''[[BE.109:Bio-material engineering/Panning for gold binders \| Day 2: Optimizing panning]]'''

-	'''[[BE.109:Bio-material engineering/~~Screening library and optimizing panning~~ \| Day 3: ~~Screening library and optimizing panning~~]]'''	+	'''[[BE.109:Bio-material engineering/Checking gold binders \| Day 3: Rescreening gold binders]]'''

-	'''[[BE.109:Bio-material engineering/~~Checking~~ gold ~~binders~~ \| Day 4: ~~Checking~~ gold ~~binders~~]]'''	+	'''[[BE.109:Bio-material engineering/PCR of gold binding candidates \| Day 4: PCR of gold binding candidates]]'''

-	'''[[BE.109~~:Bio-material engineering/PCR of gold binding candidates~~ \| Day 5: ~~PCR of gold binding candidates~~]]'''	+	'''[[http://openwetware.org/wiki/BE.109%3APresenting_your_work \| Day 5: Student presentations]]'''

	'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''		'''[[BE.109:Bio-material engineering/Sequence analysis \| Day 6: Sequence analysis]]'''