BE.180:Assignment1

Assignment PDF

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Parts

• Promoter
• RBS
• ORF
• Terminator
• Barcode

Questions and Clarifications

• Is it significant that the barcode is CAPS and the other parts are lower case?
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• Can an ORF be any length over 50, or should its length be a multiple of some small integer?
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• Does the ORF include the start ATG and stop TAA? Suppose the DNA string is "ATG...TAA": is the ORF "..." or "ATG..." or "ATG...TAA" or "...TAA"?
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• Can ORFs overlap? Suppose the DNA string is "ATG...TAAxxxTAA". The first ORF is obviously (modulo previous question) "ATG...TAA". Is "ATG...TAAxxxTAA" also an ORF? It meets the specification of "a string starting with ATG and ending with TAA". One could imagine a similar situation with overlapping starting tags: "ATG...ATGxxxTAA" might have both "ATG...ATGxxxTAA" and "ATGxxxTAA".
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