BE.180:SecondOrderBinding: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 14: | Line 14: | ||
<center><math>0=-k_{on}*[A]*[B]-k_{off}*[A:B]</math></center> | <center><math>0=-k_{on}*[A]*[B]-k_{off}*[A:B]</math></center> | ||
*Solve for <math>K_D</math>, the dissociation constant. | *Solve for <math>K_D</math>, the dissociation constant. | ||
<center><math>K_D = k_{off}/k_{on} = [A][B] | <center><math>K_D = k_{off}/k_{on} = /frac{[A][B]}{[A:B]}</math></center> |
Revision as of 17:48, 20 March 2006
Second Order Binding (of two things)
Givens:
- A physical interaction between molecules A and B.
- A system that contains some concentration of molecules A and B.
Tasks:
- Compute the steady state concentrations of free A, free B, and the A:B complex.
Approach:
- Write differential equation for change in A:B over time.
- Solve equation at steady state (that is, no change in concentration of the A:B complex.
- Solve for [math]\displaystyle{ K_D }[/math], the dissociation constant.