BE.180:SecondOrderBinding: Difference between revisions
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Givens: | Givens: | ||
*A physical interaction between molecules '''A''' and '''B'''. | *A physical interaction between molecules '''A''' and '''B'''. | ||
*A system that contains some initial concentration of molecules '''A''' and '''B''' | *A system that contains some initial concentration of molecules '''A''' and '''B''' (<math>A_0</math> and <math>B_0</math>, respectively). | ||
Tasks: | Tasks: |
Revision as of 17:50, 20 March 2006
Second Order Binding (of two things)
Givens:
- A physical interaction between molecules A and B.
- A system that contains some initial concentration of molecules A and B ([math]\displaystyle{ A_0 }[/math] and [math]\displaystyle{ B_0 }[/math], respectively).
Tasks:
- Compute the steady state concentrations of free A, free B, and the A:B complex.
Approach:
- Write differential equation for change in A:B over time.
- Solve equation at steady state (that is, no change in concentration of the A:B complex.
- Solve for [math]\displaystyle{ K_D }[/math], the dissociation constant.