BE.180:SecondOrderBinding: Difference between revisions
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<center>Equation 2: <math>[A_0] = [A] + [A:B]</math></center> | <center>Equation 2: <math>[A_0] = [A] + [A:B]</math></center> | ||
<center>Equation 3: <math>[B_0] = [B] + [A:B]</math></center> | <center>Equation 3: <math>[B_0] = [B] + [A:B]</math></center> | ||
*Note system of three unknowns with three equations! Solve for unknowns '''A''', '''B''', and '''A:B'''. | *Note system of three unknowns with three equations (1-3 above)! Solve for unknowns '''A''', '''B''', and '''A:B''' (takes you through a quadratic). |
Revision as of 18:08, 20 March 2006
Second Order Binding (of two things)
Givens:
- A physical interaction between molecules A and B.
- A system that contains some initial concentration of molecules A and B ([math]\displaystyle{ A_0 }[/math] and [math]\displaystyle{ B_0 }[/math], respectively).
Tasks:
- Compute the steady state concentrations of free A, free B, and the A:B complex.
Approach:
- Write differential equation for change in A:B over time.
- Solve equation at steady state (that is, no change in concentration of the A:B complex.
- Solve for [math]\displaystyle{ K_D }[/math], the dissociation constant.
- Note constraints on system due to conservation of mass.
- Note system of three unknowns with three equations (1-3 above)! Solve for unknowns A, B, and A:B (takes you through a quadratic).