BIO254:Transcription: Difference between revisions
No edit summary |
|||
Line 38: | Line 38: | ||
===Activation of CREB=== | ===Activation of CREB=== | ||
[[Image:test.jpg]] | [[Image:test.jpg]] | ||
[http://www.biocarta.com/pathfiles/m_crebPathway.gif Image of CREB Pathway (Biocarta)] | |||
==History== | ==History== |
Revision as of 09:02, 23 October 2006
Introduction
In activity-dependent transcription, a postsynaptic neuron changes its transcription of specific genes in response to synaptic activity. Both long-term and short-term changes are produced by synaptic activity. Long-term changes, such as synaptic plasticity and neuronal development, are often caused by changes in gene expression.
General Mechanism for Activity-Dependent Transcription
Following are the steps between the neuronal activity and the changes in gene expression:
A) Neuronal Activity
The presynaptic neuron excites or inhibits the postsynaptic neuron using neurotransmitters, such as glutamate or GABA.
B) Postsynaptic Response
The neurotransmitter activates metabotropic and/or ionotropic receptors activating G-protein/second messenger systems or producing ionic currents, respectively.
C) Signaling Pathways
Signaling proteins are activated (phosporylated/dephosphorylated) by either the G-protein pathways or the pathways affected by ionic concentrations (usually Ca2+ dependent). These pathways are often referred to as cascades because of the multiple proteins phosphorylated/dephosphorylated sequentially in a pathway.
D) Nuclear Localization
An activated protein enters the nucleus and activates/inactivates an activating or repressing transcription factor. In some cases, the protein that enters the nucleus is itself an activated transcription factor which goes directly and binds to its specific DNA sequence.
E) Transcription Factor Binding/Unbinding to DNA
The transcription factor then binds to or breaks away from the DNA, depending on whether the transcription factor was activated or inactivated, respectively.
F) Gene expression Increased/Decreased
Depending on whether the transcription factor is activated or inactivated and whether the transcription factor itself activates or represses gene expression, the transcription of the target gene(s) will be changed.
Examples of Pathways that Affect Transcription
Activation of CREB
Image of CREB Pathway (Biocarta)
History
Notes
This article has focused on activity-dependent transcription in neurons. Activity-dependent transcription occurs in various excitable cells, such as muscle cells (ie, Wamhoff et. al. 2006).
References
Wamhoff BR, Bowles DK, Owens GK. Excitation-transcription coupling in arterial smooth muscle. Circ Res. 2006 Apr 14;98(7):868-78.
Squire LR, et al. Fundamental Neuroscience. Academic Press, Boston, 2003.
Recent updates to the site:
- N
- This edit created a new page (also see list of new pages)
- m
- This is a minor edit
- b
- This edit was performed by a bot
- (±123)
- The page size changed by this number of bytes
28 March 2024
04:35 | Pecreaux diffhist +776 Cedre-PecreauxLab talk contribs (→News!) |
04:33 | Upload log Cedre-PecreauxLab talk contribs uploaded File:Announcement AI ANR MICENN EN.pdf |
26 March 2024
14:57 | Schumer lab: Commonly used workflows diffhist −64 Tododge talk contribs (→Genome assembly from PacBio HiFi data) |
|
N 00:34 | Orgenizing the data from the LCMS mechine 2 changes history +410 [Insect Nutrition and Metabolism (2×)] | |||
|
00:34 (cur | prev) +219 Insect Nutrition and Metabolism talk contribs | ||||
N |
|
00:31 (cur | prev) +191 Insect Nutrition and Metabolism talk contribs (Created page with "* In order to organize the data for further analysis , we use script in R * Please go over the presentations File:presentation1.ppt, File:presentation2.ppt, File:presentation2.ppt") |
00:28 | Laboratory for insect nutrition and metabolomics diffhist +48 Insect Nutrition and Metabolism talk contribs |
|
00:19 | (Upload log) [Insect Nutrition and Metabolism (4×)] | |||
|
00:19 Insect Nutrition and Metabolism talk contribs uploaded File:Standard curve.png | ||||
|
00:10 Insect Nutrition and Metabolism talk contribs uploaded File:Melting curve.png | ||||
|
00:08 Insect Nutrition and Metabolism talk contribs uploaded File:Melting curve2.png | ||||
|
00:05 Insect Nutrition and Metabolism talk contribs uploaded File:Meting curve.png |
|
00:17 | Designing and testing the qPCR primers 7 changes history +1,234 [Insect Nutrition and Metabolism (7×)] | |||
|
00:17 (cur | prev) +465 Insect Nutrition and Metabolism talk contribs | ||||
|
00:14 (cur | prev) +768 Insect Nutrition and Metabolism talk contribs | ||||
|
00:11 (cur | prev) +1 Insect Nutrition and Metabolism talk contribs | ||||
|
00:09 (cur | prev) +1 Insect Nutrition and Metabolism talk contribs Tag: Manual revert | ||||
|
00:07 (cur | prev) −1 Insect Nutrition and Metabolism talk contribs | ||||
|
00:06 (cur | prev) +1 Insect Nutrition and Metabolism talk contribs | ||||
|
00:03 (cur | prev) −1 Insect Nutrition and Metabolism talk contribs |
25 March 2024
23:19 | Upload log Insect Nutrition and Metabolism talk contribs uploaded File:Melting curve.pdf |
22:06 | User creation log User account Aytolgn Asnakew Melese talk contribs was created by Yar talk contribs and password was sent by email |
19:44 | BioMicroCenter:Pricing diffhist +136 Stuart S. Levine talk contribs (→VISIUM) |
18:17 | Ernesto-Perez-Rueda:Contact diffhist −4 Ernesto Perez-Rueda talk contribs |
|
11:34 | Hu:Publications 2 changes history +184 [Hugangqing (2×)] | |||
|
11:34 (cur | prev) +7 Hugangqing talk contribs | ||||
|
11:33 (cur | prev) +177 Hugangqing talk contribs |
|
11:08 | Laboratory for insect nutrition and metabolomics 2 changes history +171 [Insect Nutrition and Metabolism (2×)] | |||
|
11:08 (cur | prev) +80 Insect Nutrition and Metabolism talk contribs | ||||
|
10:56 (cur | prev) +91 Insect Nutrition and Metabolism talk contribs |
|
10:51 | CDNA synthesis 6 changes history +599 [Insect Nutrition and Metabolism (6×)] | |||
|
10:51 (cur | prev) +3 Insect Nutrition and Metabolism talk contribs | ||||
|
10:50 (cur | prev) +141 Insect Nutrition and Metabolism talk contribs | ||||
|
10:49 (cur | prev) +171 Insect Nutrition and Metabolism talk contribs | ||||
|
10:44 (cur | prev) −4 Insect Nutrition and Metabolism talk contribs | ||||
|
10:44 (cur | prev) +40 Insect Nutrition and Metabolism talk contribs | ||||
|
10:35 (cur | prev) +248 Insect Nutrition and Metabolism talk contribs |
|
08:58 | Ernesto-Perez-Rueda:Publications 2 changes history +28 [Ernesto Perez-Rueda (2×)] | |||
|
08:58 (cur | prev) +1 Ernesto Perez-Rueda talk contribs | ||||
|
08:58 (cur | prev) +27 Ernesto Perez-Rueda talk contribs |
|
01:05 | Hexlab:Publications 2 changes history +344 [Ximiao He (2×)] | |||
|
01:05 (cur | prev) −6 Ximiao He talk contribs | ||||
|
01:04 (cur | prev) +350 Ximiao He talk contribs |
24 March 2024
|
20:51 | Schumer lab: Commonly used workflows 6 changes history +1,735 [Tododge (6×)] | |||
|
20:51 (cur | prev) −1 Tododge talk contribs (→Genome assembly from PacBio HiFi data) | ||||
m |
|
20:50 (cur | prev) +201 Tododge talk contribs (→Genome assembly from PacBio HiFi data) | |||
m |
|
20:47 (cur | prev) −7 Tododge talk contribs (→Genome assembly from PacBio HiFi data) | |||
m |
|
20:46 (cur | prev) +3 Tododge talk contribs (→Genome assembly from PacBio HiFi data) | |||
m |
|
20:45 (cur | prev) +1,477 Tododge talk contribs (→Genome assembly from PacBio HiFi data) | |||
m |
|
20:18 (cur | prev) +62 Tododge talk contribs (→Genome assembly from PacBio HiFi data) |