BIOL1220/S10 - Revision history

Timothy G. Johnstone: /* Announcements */

Timothy G. Johnstone — Tue, 20 Apr 2010 17:13:09 GMT

Announcements

←Older revision		Revision as of 17:13, 20 April 2010
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	===Announcements===		===Announcements===
-	~~Short updates~~	+	4/20/10:
-	~~Ethan~~ - the ~~gas vesicles; Jen - application~~ of	+	Next Tuesday we will have our final, super hard quiz - largely from the
-	~~Hill equation; Shuba~~ - ~~PCR ligation approach~~	+	oral presentations these past two weeks. Make sure you enter your
		+	presentation slides on the website. The other part of the quiz will be
		+	project design - just as you are doing. That is - how do you
		+	create/design/test a new process.

-	~~Tuesday Feb 22nd~~	+	Final project presentations begin next week - we have scheduled 3 days
-	~~No class~~ - ~~long weekend.~~	+	for presentations, 20 minutes each, leaving time for discussion and
-		+	feedback on your project. The presentation days are April 27th, 29th,
-	~~Thursday February 24th~~	+	and May 4th. Hopefully this conversation will help you in construction
-	The ~~bacterium (Gary)~~	+	of your written work, which is due May 6th. Please consider what day
-	~~Human biome~~	+	might work best for your oral presentation, and I will have a signup
-	~~PCR techniques~~	+	sheet in class today.
-	~~Synthetic DNA technology~~	+
-		+
-	~~Tuesday March 2nd~~	+
-	~~My (~~your~~) proposed project~~ for ~~the course~~	+

	===The Course===		===The Course===

Timothy G. Johnstone: /* Announcements */

Timothy G. Johnstone — Tue, 23 Feb 2010 23:44:20 GMT

Announcements

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	Ethan - the gas vesicles; Jen - application of		Ethan - the gas vesicles; Jen - application of
	Hill equation; Shuba - PCR ligation approach		Hill equation; Shuba - PCR ligation approach
-
-	~~Thursday Feb 18th~~
-	~~Meet in lab for plasmid DNA isolation, DNA gel electrophoresis,~~
-	~~DNA transformation and cell plating.~~
-	~~Research background topic presentations 4-6.~~
-
-	~~Friday Feb 19th~~
-	~~Follow-up and analysis of transformation efficiencies.~~
-	~~On your own DNA digestion, gel electrophoresis etc.~~

	Tuesday Feb 22nd		Tuesday Feb 22nd

Timothy G. Johnstone: /* Announcements */

Timothy G. Johnstone — Tue, 16 Feb 2010 23:26:53 GMT

Announcements

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	Friday Feb 19th		Friday Feb 19th
-	Follow-up and analysis of transformation efficiencies. On your own DNA digestion, gel electrophoresis etc.	+	Follow-up and analysis of transformation efficiencies.
		+	On your own DNA digestion, gel electrophoresis etc.

	Tuesday Feb 22nd		Tuesday Feb 22nd
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	Tuesday March 2nd		Tuesday March 2nd
-	My (your) proposed project for the course ~~(further discussion on this during class).~~	+	My (your) proposed project for the course

	===The Course===		===The Course===

Timothy G. Johnstone at 23:26, 16 February 2010

Timothy G. Johnstone — Tue, 16 Feb 2010 23:26:26 GMT

←Older revision		Revision as of 23:26, 16 February 2010
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	===Announcements===		===Announcements===
-	~~Announcements will go here~~	+	Short updates
		+	Ethan - the gas vesicles; Jen - application of
		+	Hill equation; Shuba - PCR ligation approach
		+
		+	Thursday Feb 18th
		+	Meet in lab for plasmid DNA isolation, DNA gel electrophoresis,
		+	DNA transformation and cell plating.
		+	Research background topic presentations 4-6.
		+
		+	Friday Feb 19th
		+	Follow-up and analysis of transformation efficiencies. On your own DNA digestion, gel electrophoresis etc.
		+
		+	Tuesday Feb 22nd
		+	No class - long weekend.
		+
		+	Thursday February 24th
		+	The bacterium (Gary)
		+	Human biome
		+	PCR techniques
		+	Synthetic DNA technology
		+
		+	Tuesday March 2nd
		+	My (your) proposed project for the course (further discussion on this during class).

	===The Course===		===The Course===

Timothy G. Johnstone: /* Synthetic Biological Systems in Theory and Practice */

Timothy G. Johnstone — Tue, 16 Feb 2010 18:31:36 GMT

Synthetic Biological Systems in Theory and Practice

←Older revision		Revision as of 18:31, 16 February 2010
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	Professor Gary Wessel		Professor Gary Wessel
		+
		+	===Announcements===
		+	Announcements will go here

	===The Course===		===The Course===

Timothy G. Johnstone at 02:02, 16 February 2010

Timothy G. Johnstone — Tue, 16 Feb 2010 02:02:44 GMT

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Timothy G. Johnstone at 02:01, 16 February 2010

Timothy G. Johnstone — Tue, 16 Feb 2010 02:01:56 GMT

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-	<div style="padding: 10px; width: 720px; border: 5px solid #~~000000~~;">	+	<div style="padding: 10px; width: 720px; border: 5px solid #B3CD4E;">

	==Synthetic Biological Systems in Theory and Practice==		==Synthetic Biological Systems in Theory and Practice==

Timothy G. Johnstone at 02:01, 16 February 2010

Timothy G. Johnstone — Tue, 16 Feb 2010 02:01:21 GMT

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	==Synthetic Biological Systems in Theory and Practice==		==Synthetic Biological Systems in Theory and Practice==

Timothy G. Johnstone: /* The Course */

Timothy G. Johnstone — Tue, 16 Feb 2010 01:40:40 GMT

The Course

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	Every new wiki page that you create should be named BIOL1220/S10:Name of the page		Every new wiki page that you create should be named BIOL1220/S10:Name of the page
		+
	To include the header at the top of the page, include {{Template:OWW.101}} with the asterisks removed		To include the header at the top of the page, include {{Template:OWW.101}} with the asterisks removed

Timothy G. Johnstone at 01:40, 16 February 2010

Timothy G. Johnstone — Tue, 16 Feb 2010 01:40:09 GMT

←Older revision		Revision as of 01:40, 16 February 2010
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	===The Course===		===The Course===
-	Synthetic Biological Systems in Theory and Practice is a multidisciplinary laboratory, ~~lecture~~, and discussion course that combines several areas of science and engineering to give students a solid foundation in the cutting edge fields of contemporary molecular biology and synthetic biological engineering. ~~Students will pose important hypotheses~~, ~~and learn the experimental technology necessary to test them~~. ~~These technologies will include PCR protocols~~, ~~protein expression~~, ~~antibody generation~~, ~~and fluorescent protein reporters~~.	+	Synthetic Biological Systems in Theory and Practice is a multidisciplinary laboratory, lecture, and discussion course that combines several areas of science and engineering to give students a solid foundation in the cutting edge fields of contemporary molecular biology and synthetic biological engineering. Students will pose important hypotheses, and learn the experimental technology necessary to test them. These technologies will include PCR protocols, protein expression, antibody generation, and fluorescent protein reporters.

-	~~The field of synthetic biology is blossoming in in creative ways to design new cellular functions for research and applied functions~~. ~~It builds on recent work in systems biology which involves the modeling of biological systems~~, ~~but goes further in that it emphasizes the standardization of biological parts that fit together to make complex systems~~. This aspect of the course will combine discussions of the theory of the field with research engineering projects to give students the best possible tools for understanding and applying research in synthetic biology.	+	The field of synthetic biology is blossoming in in creative ways to design new cellular functions for research and applied functions. It builds on recent work in systems biology which involves the modeling of biological systems, but goes further in that it emphasizes the standardization of biological parts that fit together to make complex systems. This aspect of the course will combine discussions of the theory of the field with research engineering projects to give students the best possible tools for understanding and applying research in synthetic biology.
-		+
-	~~Biol1220 is a research course~~. ~~That is~~, ~~a discovery course~~, ~~a course in how one poses and tests hypotheses to learn answers that are yet unknown~~. ~~As a consequence~~, ~~scheduling throughout the semester will change as a result of the experimental outcome~~. ~~Further~~, ~~no one textbook~~, ~~reference~~, ~~or reading list will suffice~~. ~~Instead~~, ~~under guidance of the instructor~~, students will be put into an environment of having to identify the literature necessary to understanding the biology and the technology in order to carry out the chosen experiments. ~~Background references useful for this course include the following~~:	+	Biol1220 is a research course. That is, a discovery course, a course in how one poses and tests hypotheses to learn answers that are yet unknown. As a consequence, scheduling throughout the semester will change as a result of the experimental outcome. Further, no one textbook, reference, or reading list will suffice. Instead, under guidance of the instructor, students will be put into an environment of having to identify the literature necessary to understanding the biology and the technology in order to carry out the chosen experiments. Background references useful for this course include the following:
	Recombinant DNA: Genes and Genomics: A Short Course by James D. Watson, Richard M. Myers, Amy A. Caudy, and Jan A. Witkowski		Recombinant DNA: Genes and Genomics: A Short Course by James D. Watson, Richard M. Myers, Amy A. Caudy, and Jan A. Witkowski
	Molecular Biology of the Cell, Fifth Edition by Bruce Alberts, Alexander Johnson, Julian Lewis, and Martin Raff		Molecular Biology of the Cell, Fifth Edition by Bruce Alberts, Alexander Johnson, Julian Lewis, and Martin Raff
-		+
-	~~The course will meet formally twice per week with focused lab sessions interspersed with discussion and seminar formats~~. ~~Students will be responsible for presentations of technique~~, ~~laboratory research results~~, ~~biological mechanisms~~, ~~and literature summaries~~. ~~Research will be based on creative~~, ~~synthetic biological projects designed as a result of discussions amongst the student and faculty~~.	+	The course will meet formally twice per week with focused lab sessions interspersed with discussion and seminar formats. Students will be responsible for presentations of technique, laboratory research results, biological mechanisms, and literature summaries. Research will be based on creative, synthetic biological projects designed as a result of discussions amongst the student and faculty.
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