BIOL368/F11:Class Journal Week 7

Robert W. Arnold

1. The easiest part of the article was that it continued on the topic of HIV. Now that we have some background and general information, it is easier to get more in depth on a molecular level. I tend to enjoy molecular and it was interesting to come from a project like Markham that dealt with things on a broad scale to something very specified like the V3 region of the gp120 protein.
2. I found the article extremely challenging to read. Hopefully, it is due to lack of sleep and general tiredness as it has been a stressful week so far. I could not get a good understanding of the paper due to the repetition of antibodies, loop regions, residues, conformations, etc. I will definitely need more time and some possible extracurricular research to fully grasp this article. I plan to reread the article and go back over my outline in the next few days.
3. My new question is about the gp160 protein, the one that codes for gp41 and gp 120. I wonder if there could an antibody that could suppress the gp160 from even encoding gp120. If there were, I would be curious to see what the effects would be, whether or not the virus would use other means to attach to receptors. If gp120 is responsible for binding and it is no longer made, I would assume the virus would not be able to infect healthy cells.

Robert W Arnold 19:37, 12 October 2011 (EDT)

Isaiah M. Castaneda

1. Perhaps the main factors in being able to navigate through the article were having already been introduced to the HIV-1 virus through previous assignments and Dr. Dahlquist’s pre-assignment explanations. Another helpful factor was that within the article, there were many vibrant animations with meaningful captions and labels. They really helped to visualize what was being discussed in the paper. The wording/grammar was easy to follow as well.
2. The frequent in-text citations were a bit of an annoyance. It felt similar to enjoying a smooth drive, until a barrage of discourteous drivers cut you off. Naturally, I understand the importance of citing sources throughout an article, but coming across so many of them was just the first thing I recall peeving me. I have always preferred an endnotes style of in-text citing. Similar to what is difficult in any situation, there were still plenty of things that I was unfamiliar with. There was a vast amount of programs, procedures, and jargon that I had not previously heard of. This is what contributed to the bulk of the difficulty in reading the article.
3. A question that the article inspired is, if a V3-based fusion inhibitor were to be created, what are the odds of the virus’ success? Do we know if such a specific inhibitor is enough to conquer HIV-1, or does the virus have other means of binding that it my utilize?

Isaiah M. Castaneda 22:34, 16 October 2011 (EDT)

Alex A. Cardenas

1. The easiest aspect of reading/understanding my journal club article was the step by step details that it provided in order to get to the conclusion. I took a step back and tried to look at the bigger picture of what was going on, and writing it down on a piece of paper also helped.
2. The most difficult aspect of reading/understanding my journal club article was that there was so much information. There are so many little details and didn't know if I should include them in my outline. Another hard part was trying to understand all the different numbers and abbreviations because once a protein or enzyme was named, they almost always had an abbreviation for the rest of the reading.
3. What biological functions does the flexibility of the V3 loop conformation possess?

Alex A. Cardenas Alex A. Cardenas 17:10, 17 October 2011 (EDT)