# BIOL398-01/S11:Class Journal Week 4

(Difference between revisions)
 Revision as of 17:56, 6 February 2011 (view source)m (→James C. Clements' Journal Entry: Signature)← Previous diff Revision as of 18:22, 6 February 2011 (view source) (Sarah's entry!! :))Next diff → Line 19: Line 19: === [[User:Sarah Carratt|Sarah Carratt's]] Journal Entry === === [[User:Sarah Carratt|Sarah Carratt's]] Journal Entry === - # + # The purpose of this assignment was to practice using the Michaelis Menten model and Matlab. - # + # Finding Vmax and K was easiest for me. - # + # Matlab remains the hardest part of the assignment for me - # + # I keep getting error messages in Matlab that I have trouble correcting. It can be really frustrating and I feel like I won't ever understand it on the first try. - # + + [[User:Sarah Carratt|Sarah Carratt]] 17:22, 6 February 2011 (EST) === [[User:Carmen E. Castaneda|Carmen E. Castaneda's]] Journal Entry === === [[User:Carmen E. Castaneda|Carmen E. Castaneda's]] Journal Entry ===

## Instructions

### Formatting

• Link to your journal entry from your user page.
• Link back from the journal entry to your user page.
• Sign your portion of the journal with the standard wiki signature shortcut (`~~~~`).
• Add the "BIOL398-01/S11" category to the end of the wiki page (if someone has not already done so).

### Reflection

1. What was the purpose of this assignment?
2. What aspect of this assignment came most easily to you?
3. What aspect of this assignment was the most challenging for you?
4. What (yet) do you not understand?

## Class Responses

### Sarah Carratt's Journal Entry

1. The purpose of this assignment was to practice using the Michaelis Menten model and Matlab.
2. Finding Vmax and K was easiest for me.
3. Matlab remains the hardest part of the assignment for me
4. I keep getting error messages in Matlab that I have trouble correcting. It can be really frustrating and I feel like I won't ever understand it on the first try.

Sarah Carratt 17:22, 6 February 2011 (EST)

### James C. Clements' Journal Entry

1. The purpose of this assignment was to learn more about the effect of reaction rates on enzyme kinetics using the Michaelis Menten model. The value for the reaction from the enzyme-substrate complex going back into enzyme and substrate was adjusted, as was the constant for the reaction going from enzyme-substrate complex into product.
2. The Matlab programming came most easily to me.
3. The terminology section was the most difficult for me to understand. I got a little hung up on how to define a chemostat reactor and how to define the Lineweaver-Burk experiment.
4. I don't understand why we're using the Lineweaver-Burk plotting method for measuring enzyme kinetics. The Hanes-Woolf plot seems to be regarded as a superior method. Furthermore, the use of such plots has been made obsolete by nonlinear regression techniques; I'm sure that MatLab is capable of doing a nonlinear regression and giving us the parameters that we're looking for.

James C. Clements 16:56, 6 February 2011 (EST)