BIOL398-01/S11:Week 4 - Revision history

Kam D. Dahlquist: /* Applying Michealis-Menten Models */ fixed spelling

2011-02-08T00:07:41Z

Applying Michealis-Menten Models: fixed spelling

←Older revision		Revision as of 00:07, 8 February 2011
Line 22:		Line 22:
	# Exponential growth.		# Exponential growth.

-	=== Applying ~~Michealis~~-Menten Models ===	+	=== Applying Michaelis-Menten Models ===

	* Consider a single substrate being converted to product.		* Consider a single substrate being converted to product.

Kam D. Dahlquist: /* Terminology */ fixed spelling

2011-02-08T00:06:57Z

Terminology: fixed spelling

←Older revision		Revision as of 00:06, 8 February 2011
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	* Explain the following terms in your own words.		* Explain the following terms in your own words.
	# The Michaelis-Menten model of enzyme kinetics.		# The Michaelis-Menten model of enzyme kinetics.
-	# The experiment required for the Lineweaver-Burk approach to estimating parameters for ~~Michealis~~-Menten.	+	# The experiment required for the Lineweaver-Burk approach to estimating parameters for Michaelis-Menten.
	# Chemostat reactor.		# Chemostat reactor.
	# Exponential growth.		# Exponential growth.

Ben G. Fitzpatrick: added problem

2011-02-04T00:51:18Z

added problem

←Older revision		Revision as of 00:51, 4 February 2011
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	=== Estimating Michaelis-Menten Parameters ===		=== Estimating Michaelis-Menten Parameters ===
-	* Consider	+	* Consider the following data:
		+
		+	[S] = 11.0, 16.7, 20.0, 25.0, 33.3, 50.0
		+
		+	V = 0.00952, 0.01111, 0.01282, 0.01515, 0.01852, 0.02128
		+
		+	Apply the Lineweaver-Burk technique to determine Vmax and K.

	== Shared Journal Assignment ==		== Shared Journal Assignment ==

Ben G. Fitzpatrick: bgf added homework assgt 4

2011-02-03T16:18:36Z

bgf added homework assgt 4

New page

{{BIOL398-01/S11}}

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'''This journal entry is due on Monday, February 07 at midnight PST''' ''(Sunday night/Monday morning)''. NOTE that the server records the time as Eastern Standard Time (EST). Therefore, midnight will register as 03:00.

== Individual Journal Assignment ==

* Store this journal entry as "''username'' Week 4" (i.e., this is the text to place between the square brackets when you link to this page).
* Create the following set of links. (HINT: you can do all of this easily by adding them to your template and then using the template on your pages.)
** Link to your journal entry from your user page.
** Link back from your journal entry to your user page.
** Link to this assignment from your journal entry.
** Don't forget to add the "BIOL398-01/S11" category to the end of your wiki page.

=== Terminology ===

* Explain the following terms in your own words.
# The Michaelis-Menten model of enzyme kinetics.
# The experiment required for the Lineweaver-Burk approach to estimating parameters for Michealis-Menten.
# Chemostat reactor.
# Exponential growth.

=== Applying Michealis-Menten Models ===

* Consider a single substrate being converted to product.
''E'' + ''S'' ↔ ''ES'' → ''E'' + ''P''
with an initial substrate concentration of 1.0 and an initial enzyme concentration of 0.05. Use the Michaelis-Menten matlab model to explore the following.
# Set k1 = 2.0, k-1 = 0.0. Plot the substrate dynamics for k2 = 1.0, k2 = 2.0, k2 = 5.0, k2 = 10.0 all on the same graph to see the effect of this parameter.
# Set k1 = 2.0, k-1 = 1.0. Plot the substrate dynamics for k2 = 1.0, k2 = 2.0, k2 = 5.0, k2 = 10.0 all on the same graph to see the effect of this parameter.
# Set k1 = 2.0, k-1 = 5.0. Plot the substrate dynamics for k2 = 1.0, k2 = 2.0, k2 = 5.0, k2 = 10.0 all on the same graph to see the effect of this parameter.
You may use the matlab code provided at [https://lionshare.lmu.edu/xythoswfs/webview/fileManager.action?x=y&shareLogin=false&stk=7B20EDA3576916A my lionshare folder].

=== Estimating Michaelis-Menten Parameters ===
* Consider

== Shared Journal Assignment ==

* Store your journal entry in the shared [[BIOL398-01/S11:Class Journal Week 4 | Class Journal Week 4]] page. If this page does not exist yet, go ahead and create it (congratulations on getting in first ''':)''' )
* Link to your journal entry from your user page.
* Link back from the journal entry to your user page.
* Sign your portion of the journal with the standard wiki signature shortcut (<code><nowiki>~~~~</nowiki></code>).
* Add the "BIOL398-01/S11" category to the end of the wiki page (if someone has not already done so).

=== Reflection ===

# What was the purpose of this assignment?
# What aspect of this assignment came most easily to you?
# What aspect of this assignment was the most challenging for you?
# What (yet) do you not understand?
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